Role of glucose side chains with serotype-specific polysaccharide in the cariogenicity of Streptococcus mutans
Role of glucose side chains with serotype-specific polysaccharide in the cariogenicity of Streptococcus mutans
复制标题
DOI：
10.1159/000084831
复制
发表时间：
2005-01-01
期刊：
影响因子：
4.2
通讯作者：
Ooshima, T
中科院分区：
文献类型：
--
作者：
Nakano, K;Nomura, R;Ooshima, T
此前，我们从人类血液和口腔样本中分离并鉴定了一株新的变形链球菌（k血清型），发现k血清型菌株的血清学特性与glua灭活的MT8148突变株（MT8148GD）相似。与亲本菌株相比，MT8148GD对玻璃表面的蔗糖依赖性粘附、对唾液包被的羟基磷灰石的蔗糖非依赖性粘附、葡聚糖结合活性和细胞相关葡萄糖基转移酶（GTF）活性显著降低。此外，Western blot分析显示，与MT8148相比，血清型k菌株的GTFB和GTFC表达降低，尽管MT8148GD和血清型k口服分离物在大鼠中的致龋活性与MT8148相似。我们得出结论，变形链球菌血清型特异性多糖中的葡萄糖侧链缺陷可能与其致龋性相关，尽管其程度低于其他主要表面蛋白。版权所有(C) 2005 S. Karger AG，巴塞尔。
Previously, we isolated and characterized a new Streptococcus mutans strain ( serotype k) from human blood and oral cavity samples, and found that the serological properties of serotype k strains were similar to those of a gluA-inactivated mutant strain of MT8148 (MT8148GD). MT8148GD showed significantly lower sucrose-dependent adhesion to glass surfaces, sucrose-independent adhesion to saliva-coated hydroxyapatite, dextran-binding activity, and cell-associated glucosyltransferase (GTF) activity than the parent strain. Further, Western blot analysis revealed reduced GTFB and GTFC expression in serotype k strains as compared to MT8148, though the caries-inducing activities of MT8148GD and a serotype k oral isolate in rats were similar to that of MT8148. We conclude that a glucose side-chain defect in the serotype-specific polysaccharide of S. mutans may be associated with its cariogenicity, though to a lesser extent than its other major surface proteins. Copyright (C) 2005 S. Karger AG, Basel.