A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019
A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019
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DOI：
10.1093/infdis/jiaa243
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发表时间：
2020-07-15
影响因子：
6.4
通讯作者：
Wang, De-qiang
中科院分区：
文献类型：
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作者：
Cai, Xue-fei;Chen, Juan;Wang, De-qiang
背景严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是一种新型β-冠状病毒，可引起严重的肺炎，并已迅速蔓延到地球仪各地。与SARS-CoV-2感染相关的疾病被命名为2019冠状病毒病（COVID-19）。到目前为止，实时逆转录聚合酶链反应（RT-PCR）是唯一能够确认这种感染的测试。然而，RT-PCR的准确性取决于几个因素;这些因素的变化可能会显着降低检测的灵敏度。在这项研究中，我们开发了一种基于肽的发光免疫测定法，检测免疫球蛋白（IG）G和IgM。通过评估健康和感染患者血清中除SA RS-CoV-2以外的病原体来确定检测临界值。结果。为了评估检测性能，我们检测了确诊患者血清中的IgG和IgM。IgG和IgM的阳性率分别为71.4%和57.2%。因此，将我们的免疫测定与实时RT-PCR相结合可能会提高COVID-19的诊断准确性。
Background. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel beta-coronavirus, causes severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named coronavirus disease 2019 (COVID-19). To date, real-time reverse-transcription polymerase chain reaction (RT-PCR) is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection.Methods. In this study, we developed a peptide-based luminescent immunoassay that detected immunoglobulin (Ig)G and IgM. The assay cutoff value was determined by evaluating the sera from healthy and infected patients for pathogens other than SA RS-CoV-2.Results. To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively.Conclusions. Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19.