EFFECT OF STRAIN OF STAPHYLOCOCCUS-AUREUS ON SYNERGISM WITH CANDIDA-ALBICANS RESULTING IN MOUSE MORTALITY AND MORBIDITY
EFFECT OF STRAIN OF STAPHYLOCOCCUS-AUREUS ON SYNERGISM WITH CANDIDA-ALBICANS RESULTING IN MOUSE MORTALITY AND MORBIDITY
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DOI：
10.1128/iai.42.1.285-292.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
CARLSON, E
中科院分区：
文献类型：
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作者：
CARLSON, E
9株金黄色葡萄球菌分离自中毒性休克综合征(TSS)患者，2株来自非疾病相关来源，4株来自疾病相关来源(非TSS)。LD50和毒性休克毒素的产生，并研究了与亚致死剂量的白念珠菌和免疫接种时对小鼠死亡率和发病率的协同效应。典型的毒性休克毒素产生菌株(不含其他肠毒素)在接种I.P.时表现出以下一组独特的特征。对小鼠的致死率最低；单独接种小鼠的LD50最低(LD50为其他菌株的200倍)；与白色念珠菌和腹腔注射联合注射时，LD50降低最大(达70,000倍)；在双重注射的动物中诱导出一种不依赖剂量的暂时性死亡模式。当使用亚致死剂量时，接受疾病(TSS而不是TSS)相关金黄色葡萄球菌和白色念珠菌混合的动物会出现症状；根据菌株的不同，观察到症状上的一些差异。症状包括结膜炎；胃肠道、神经和循环异常；皮疹，随后出现脱皮和斑片性秃顶。虽然观察到动物治疗组之间的症状重叠，但某些症状(神经后遗症和点状出血)仅在接种了特定金黄色葡萄球菌菌株和白色念珠菌的动物中观察到。接受亚致死性双重剂量的动物，包括非疾病相关的金黄色葡萄球菌，没有出现症状。将表皮葡萄球菌与白色念珠菌联合接种小鼠，未观察到协同作用对发病率和死亡率的影响。
Nine S. aureus strains isolated from patients with toxic shock syndrome (TSS), 2 strains from non-disease-associated sources and 4 strains from disease (not TSS)-associated sources were characterized for i.p. LD50 and toxic shock toxin production and studied for synergistic effects on mouse mortality and morbidity when combined with a sublethal dose of C. albicans and inoculated i.p. Representative toxic shock toxin-producing strains (free of other enterotoxins) exhibited the following unique set of characteristics when inoculated i.p. into mice and compared with all other strains tested; lowest virulence when inoculated alone into mice as determined by the LD50; greatest synergistic decrease in LD50 (up to 70,000-fold as compared to up to 200-fold for other strains) when combined with C. albicans and injected i.p.; and induced a characteristic, dose-independent, temporal death pattern in dually injected animals. When sublethal dual doses were used, animals receiving disease (TSS and not TSS)-associated S. aureus in combination with C. albicans developed symptoms; some differences in symptomatologies, depending on the strain, were observed. The symptoms included conjunctivitis; gastrointestinal, neurological and circulatory abnormalities; rash followed by desquamation and patchy baldness. Although overlap in symptoms between animal treatment groups was observed, certain symptoms (neurological sequeae and petechial hemorrhages) were observed only in animals inoculated with a specific S. aureus strain combined with C. albicans. Animals receiving sublethal dual doses, which included non-disease-associated S. aureus, did not develop symptoms. When S. epidermidis was combined with C. albicans and inoculated into mice, no synergistic effects on morbidity or mortality were observed.