STU1, a suppressor of a beta-tubulin mutation, encodes a novel and essential component of the yeast mitotic spindle.
STU1, a suppressor of a beta-tubulin mutation, encodes a novel and essential component of the yeast mitotic spindle.
复制标题
DOI：
10.1083/jcb.127.6.1973
复制
发表时间：
1994-12
期刊：
影响因子：
--
通讯作者：
Huffaker TC
中科院分区：
文献类型：
--
作者：
Pasqualone D;Huffaker TC
我们已经分离到酿酒酵母中唯一编码β-微管蛋白的基因TUB2的一个冷敏感等位基因，该基因导致纺锤体微管功能的特定缺陷。在14摄氏度时，Tub2-406细胞缺乏正常的双极纺锤体，但确实组装了功能正常的细胞质微管。为了确定对纺锤体组装至关重要的蛋白质，我们筛选了tub2-406冷敏感性的抑制因子，并获得了一个新基因STU1的四个等位基因。STU1等位基因与TUB1、TUB2等位基因之间的遗传交互作用表明，Stu1p与微管发生特异的相互作用。STU1对生长是必不可少的，STU1的中断会导致纺锤体组装的缺陷，这种缺陷类似于tub2-406突变所产生的缺陷。STU1基因的核苷酸序列预测出一个174kD的蛋白质产物，与已知的蛋白质没有明显的相似性。标记了表位的Stulp与微管共存于酵母的有丝分裂纺锤体中。这些结果表明，Stulp是酵母有丝分裂纺锤体的重要组成部分。
We have isolated a cold-sensitive allele of TUB2, the sole gene encoding beta-tubulin in S. cerevisiae, that confers a specific defect in spindle microtubule function. At 14 degrees C, tub2-406 cells lack a normal bipolar spindle but do assemble functional cytoplasmic microtubules. In an attempt to identify proteins that are important for spindle assembly, we screened for suppressors of the cold-sensitivity of tub2-406 and obtained four alleles of a novel gene, STU1. Genetic interactions between stu1 alleles and alleles of TUB1 and TUB2 suggest that Stu1p specifically interacts with microtubules. STU1 is essential for growth and disruption of STU1 causes defects in spindle assembly that are similar to those produced by the tub2-406 mutation. The nucleotide sequence of the STU1 gene predicts a protein product of 174 kD with no significant similarity to known proteins. An epitope-tagged Stulp colocalizes with microtubules in the mitotic spindle of yeast. These results demonstrate that Stulp is an essential component of the yeast mitotic spindle.