Activatable Rotor for Quantifying Lysosomal Viscosity in Living Cells
Activatable Rotor for Quantifying Lysosomal Viscosity in Living Cells
复制标题
用于量化活细胞中溶酶体粘度的可激活转子
DOI：
10.1021/ja311688g
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发表时间：
2013-02-27
影响因子：
15
通讯作者：
Deng, Liezheng
中科院分区：
文献类型：
--
作者：
Wang, Lu;Xiao, Yi;Deng, Liezheng
我们已经开发了Lyso-V，第一个荧光探针的溶酶体粘度。由于Lyso-V具有溶酶体激活的荧光特性，在激光共聚焦显微镜下，Lyso-V被证明是一种理想的溶酶体示踪剂，具有高的空间和时间分辨率。更重要的是，Lyso-V显示了其在通过荧光寿命成像显微镜实时定量活细胞中溶酶体粘度变化方面的实用性。
We have developed Lyso-V, the first fluorescent probe of lysosomal viscosity. Because of its lysosome-actived fluorescence characteristics, Lyso-V has proved to be an ideal lysosomal tracer with high spatial and temporal resolution under laser confocal microscopy. More importantly, Lyso-V shows its practical applicability in real-time quantification of lysosomal viscosity changes in live cells through fluorescence lifetime imaging microscopy.