(1-Pyrene)sulfonyl azide: a fluorescent probe for measuring the transmembrane topology of acetylcholine receptor subunits.
(1-Pyrene)sulfonyl azide: a fluorescent probe for measuring the transmembrane topology of acetylcholine receptor subunits.
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(1-芘)磺酰叠氮：一种用于测量乙酰胆碱受体亚基跨膜拓扑的荧光探针。
DOI：
10.1016/0003-9861(87)90429-2
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发表时间：
1987
影响因子：
3.9
通讯作者：
Martinez-Carrion,M
中科院分区：
文献类型：
--
作者：
Clarke,J;Garcia-Borron,JC;Martinez-Carrion,M
（1-芘）磺酰叠氮化物（PySA）是一种荧光亲脂性光标记物，用于探测乙酰胆碱受体（AchR）亚基的跨膜拓扑结构。天然的、碱提取的和重组的AchR膜制剂的光标记导致所有AchR亚基的标记。然而，重组的AchR膜制剂在每摩尔AchR复合物中加入了两倍的PySA。所有AchR亚基的光标记似乎不会改变AchR介导的阳离子易位的激动剂浓度反应。
(1-Pyrene)sulfonyl azide (PySA), a fluorescent, lipophilic photolabel, was used as a probe for the transmembrane topology of the acetylcholine receptor (AchR) subunits. Photolabeling of native, alkaline-extracted, and reconstituted AchR membrane preparations resulted in the labeling of all the AchR subunits. However the reconstituted AchR membrane preparations incorporated twice as much PySA into each mole of the AchR complex. Photolabeling of all subunits of the AchR does not appear to alter the agonist concentration response of AchR-mediated cation translocation.