Low-frequency stimulation of the external globus palladium produces anti-epileptogenic and anti-ictogenic actions in rats
Low-frequency stimulation of the external globus palladium produces anti-epileptogenic and anti-ictogenic actions in rats
复制标题
低频刺激外部钯球对大鼠产生抗癫痫和抗癫痫作用
DOI：
10.1038/aps.2015.45
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发表时间：
2015-08-01
影响因子：
8.2
通讯作者：
Chen, Zhong
中科院分区：
文献类型：
--
作者：
Cheng, Hui;Kuang, Yi-fang;Chen, Zhong
目的：探讨靶向外部钯球（GPe）的脑深部电刺激对大鼠癫痫的抑制作用。方法：将双极不锈钢电极分别植入SD大鼠右侧基底外侧杏仁核和右侧GPe，诱发杏仁核点燃和脑深部电刺激。采用杏仁核点燃模型、最大电休克模型（MES）和戊四唑（PTZ）模型评价脑深部电刺激的作用。结果：低频（0.1ms，1Hz，15min）刺激GPe可使点燃过程中癫痫发作的进程减慢，后放电时程（ADD）缩短。此外，低频刺激显着降低全身性发作的发生率，抑制平均阶段，并缩短了累积ADD和全身性发作持续时间在完全点燃大鼠。此外，在PTZ模型中，低频刺激显著抑制MES诱导的癫痫发作的平均阶段，并增加全身性癫痫发作的潜伏期。高频电刺激GPe（0.1ms，130Hz，5min）对杏仁核点燃致痫无明显的抗痫作用，甚至可加重杏仁核点燃致痫。EEG分析表明，在GPe的低频刺激扭转了三角洲功率的增加，而在GPe的高频刺激没有这样的effect.Conclusion：低频刺激，而不是高频刺激，在GPe颞叶癫痫和强直性结肠全身性发作，这可能是由于干扰δ节律的治疗效果。结果表明，使用低频刺激或药物调节GPe活性可能是一种有前途的癫痫治疗方法。
Aim:To investigate the anti-epileptic effects of deep brain stimulation targeting the external globus palladium (GPe) in rats.Methods:For inducing amygdala kindling and deep brain stimulation, bipolar stainless-steel electrodes were implanted in SD rats into right basolateral amygdala and right GPe, respectively. The effects of deep brain stimulation were evaluated in the amygdala kindling model, maximal electroshock model (MES) and pentylenetetrazole (PTZ) model. Moreover, the background EEGs in the amygdala and GPe were recorded.Results:Low-frequency stimulation (0.1 ms, 1 Hz, 15 min) at the GPe slowed the progression of seizure stages and shortened the after-discharge duration (ADD) during kindling acquisition. Furthermore, low-frequency stimulation significantly decreased the incidence of generalized seizures, suppressed the average stage, and shortened the cumulative ADD and generalized seizure duration in fully kindled rats. In addition, low-frequency stimulation significantly suppressed the average stage of MES-induced seizures and increased the latency to generalized seizures in the PTZ model. High-frequency stimulation (0.1 ms, 130 Hz, 5 min) at the GPe had no anti-epileptic effect and even aggravated epileptogenesis induced by amygdala kindling. EEG analysis showed that low-frequency stimulation at the GPe reversed the increase in delta power, whereas high-frequency stimulation at the GPe had no such effect.Conclusion:Low-frequency stimulation, but not high-frequency stimulation, at the GPe exerts therapeutic effect on temporal lobe epilepsy and tonic-colonic generalized seizures, which may be due to interference with delta rhythms. The results suggest that modulation of GPe activity using low-frequency stimulation or drugs may be a promising epilepsy treatment.