Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast.
Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast.
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DOI：
10.1093/nar/gki728
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发表时间：
2005
影响因子：
14.9
通讯作者：
MacNeill SA
中科院分区：
文献类型：
--
作者：
Kim J;Robertson K;Mylonas KJ;Gray FC;Charapitsa I;MacNeill SA
复制因子C（replicationfactorC，RFC）介导的细胞核抗原负载DNA是真核生物DNA复制和修复过程中的关键步骤。在这项研究中，C-末端结构域（CTD）的大亚基的裂殖酵母RFC被证明是必不可少的，其功能在体内。在CTD中携带温度敏感性突变的细胞，rfc 1 - 44，因不完全复制的染色体而停滞，对DNA损伤剂敏感，对其他DNA复制突变体具有合成致死性，并且可以被rfc 5中的突变抑制。为了评估RFC样复合物Elg1-RFC和Ctf18-RFC对rfc1 - 44活力的贡献，编码这些复合物的大亚基的基因被删除并过表达。通过删除ctf18+、dcc 1+或ctf8+使Ctf18-RFC失活在rfc 1 - 44背景下是致命的，这表明在没有完全功能性RFC的情况下需要完全的Ctf18-RFC功能。相反，rfc1 - 44 elg1 Δ细胞是活的，并且rfc1 - 44中Elg1的过量产生是致命的，这表明当RFC功能被抑制时，Elg1-RFC起着负面作用。与此一致，elg 1+的缺失显示恢复rfc 1 - 44 ctf 18 Δ细胞的活力。
Proliferating cell nuclear antigen loading onto DNA by replication factor C (RFC) is a key step in eukaryotic DNA replication and repair processes. In this study, the C-terminal domain (CTD) of the large subunit of fission yeast RFC is shown to be essential for its function in vivo. Cells carrying a temperature-sensitive mutation in the CTD, rfc1-44, arrest with incompletely replicated chromosomes, are sensitive to DNA damaging agents, are synthetically lethal with other DNA replication mutants, and can be suppressed by mutations in rfc5. To assess the contribution of the RFC-like complexes Elg1–RFC and Ctf18–RFC to the viability of rfc1-44, genes encoding the large subunits of these complexes have been deleted and overexpressed. Inactivation of Ctf18–RFC by the deletion of ctf18+, dcc1+ or ctf8+ is lethal in an rfc1-44 background showing that full Ctf18–RFC function is required in the absence of fully functional RFC. In contrast, rfc1-44 elg1Δ cells are viable and overproduction of Elg1 in rfc1-44 is lethal, suggesting that Elg1–RFC plays a negative role when RFC function is inhibited. Consistent with this, the deletion of elg1+ is shown to restore viability to rfc1-44 ctf18Δ cells.
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影响因子：
11.4
作者：
MacNeill, SA;Moreno, S;Fantes, PA
通讯作者：
Fantes, PA
DOI：
10.1073/pnas.1434308100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Bermudez, VP;Maniwa, Y;Hurwitz, J
通讯作者：
Hurwitz, J
影响因子：
64.8
作者：
Bowman, GD;O'Donnell, M;Kuriyan, J
通讯作者：
Kuriyan, J
影响因子：
14.9
作者：
FRANCESCONI, S;PARK, H;WANG, TSF
通讯作者：
WANG, TSF
DOI：
10.1073/pnas.0437927100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Bermudez, VP;Lindsey-Boltz, LA;Sancar, A
通讯作者：
Sancar, A