MASCULINIZATION AND DEFEMINIZATION OF FEMALE RATS BY MALES LOCATED CAUDALLY IN THE UTERUS
MASCULINIZATION AND DEFEMINIZATION OF FEMALE RATS BY MALES LOCATED CAUDALLY IN THE UTERUS
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DOI：
10.1016/0031-9384(90)90299-j
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发表时间：
1990-10-01
影响因子：
2.9
通讯作者：
SLOB, AK
中科院分区：
文献类型：
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作者：
HOUTSMULLER, EJ;SLOB, AK
据推测，雌性大鼠的雄性化是由于雄性在同一个子宫角的存在。关于这一机制提出了两种假说：1)雄激素在羊膜内扩散（邻近假说）和2)雄激素通过血流运输（尾侧雄性假说）。本研究旨在验证这些假设，同时考虑到两个先前无法控制的因素：怀孕期间母亲的半子宫切除术和剖腹产分娩。孕妇在怀孕期间切除半子宫或保留完整子宫；幼崽是通过自然或剖腹产出生的。确定子宫内位置。在成年期，所有女性都被切除卵巢，并在睾酮治疗前和治疗期间检测其增加的行为和雌二醇治疗期间的前凸行为。研究发现，在子宫角尾端有雄虫的雌虫比没有雄虫的雌虫表现出更多的男性化和非女性化。相邻雄性对雌性的行为性别分化没有影响。这些结果证实了“尾侧雄性假说”而不是“邻近假说”。怀孕期间的半子宫切除术防止了“尾端男性效应”。剖宫产并不影响尾侧男性效应。
It is assumed that female rats are masculinized by the presence of males in the same uterine horn. Two hypotheses regarding the mechanism have been proposed: 1) interamniotic diffusion of androgens (contiguity hypothesis) and 2) transport of androgens via the bloodflow (caudal male hypothesis). This study was designed to test these hypotheses while taking into account two previously uncontrolled factors: hemihysterectomy of the mother during pregnancy and birth by caesarean section. Pregnant females were hemihysterectomized during pregnancy or left intact; pups were born naturally or through caesarean section. Position in utero was determined. In adulthood all females were ovariectomized and tested for mounting behavior before and during testosterone treatment and lordosis behavior during estradiol treatment. It was found that females with males located caudally in the same uterine horn were more masculinized and defeminized than females without such males. Adjacent males had no influence on the behavioral sexual differentiation of females. These results confirm the "caudal male hypothesis" rather than the "contiguity hypothesis." Hemihysterectomy during pregnancy prevented the "caudal male effect." Birth through caesarean section did not interfere with the caudal male effect.