In vivo bone regenerative effect of low-intensity pulsed ultrasound in rat calvarial defects
In vivo bone regenerative effect of low-intensity pulsed ultrasound in rat calvarial defects
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DOI：
10.1016/j.tripleo.2010.09.061
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Ito, Koichi
中科院分区：
文献类型：
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作者：
Hasuike, Akira;Sato, Shuichi;Ito, Koichi
目标。低强度脉冲超声 (LIPUS) 是骨修复过程中的生物物理干预措施。然而，使用 LIPUS 的颅骨扁平骨的愈合事件和最佳刺激设置都尚未完全了解。本研究的目的是利用体内微焦点计算机断层扫描（micro-CT）评估LIPUS对大鼠颅骨扁平骨缺损的骨再生效果。研究设计。暴露颅骨，并制备非临界尺寸的 2.7 毫米缺损。 LIPUS（1.6 MHz，重复 1.0 kHz 脉动，30 mW/cm(2) 强度）每天应用 20 分钟。使用显微 CT 和组织学检查进行图像分析来评估骨再生。结果。两周内，LIPUS 治疗的大鼠表现出 7.0% 的原始手术缺陷再骨化，而对照大鼠则表现出 3.6%。第 3 周和第 4 周时，观察到再骨化率存在显着差异（分别为 12.0% vs. 5.8% 和 18.1% vs. 9.8%；P
Objectives. Low-intensity pulsed ultrasound (LIPUS) is a biophysical intervention in a bone repair process. However, neither the healing events of the flat bones of the skull using LIPUS nor the optimal stimulation settings are fully understood. The aim of this study was to evaluate the bone regenerative effect of LIPUS in rat calvarial flat bone defects by using in vivo microfocus computerized tomography (micro-CT).Study design. The calvarium was exposed, and noncritical-sized 2.7-mm defects were prepared. LIPUS (1.6 MHz, repeating 1.0-kHz pulsation, and 30-mW/cm(2) intensity) was applied for 20 minutes daily. Bone regeneration was evaluated by image analysis using micro-CT and histologic examination.Results. Within 2 weeks, LIPUS-treated rats demonstrated 7.0% reossification of the original surgical defect, whereas control rats demonstrated 3.6%. At 3 and 4 weeks, a significant difference in the reossification ratio was observed (12.0% vs. 5.8% and 18.1% vs. 9.8%, respectively; P