Expression patterns of the erbB subfamily mRNA in canine benign and malignant mammary tumors.
Expression patterns of the erbB subfamily mRNA in canine benign and malignant mammary tumors.
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erbB 亚家族 mRNA 在犬良性和恶性乳腺肿瘤中的表达模式。
DOI：
10.1292/jvms.63.949
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
K. Kubo
中科院分区：
文献类型：
--
作者：
S. Matsuyama;Mieko Nakamura;Kumiko Yonezawa;Terumasa Shimada;Fumihito Ohashi;Yasuhiko Takamori;K. Kubo
ErbB亚家族基因，称为原癌基因，编码受体酪氨酸激酶，并与人类乳腺肿瘤发生相关。在这项研究中，我们研究了erbB亚家族mRNA的表达在两个正常的犬乳腺和12个乳腺肿瘤样本的逆转录酶偶联聚合酶链反应（RT-PCR）。每个引物组是从其它物种中erbB亚家族cDNA保守区域的核苷酸序列设计的。正常乳腺组织中不表达erbB亚家族mRNA。与此相反，所有的亚家族的mRNA表达在良性乳腺肿瘤，并观察到一个以上的一种类型的亚家族的mRNA在11个恶性乳腺肿瘤。RT-PCR扩增产物长度分别为erbB 1 380 bp、erbB 2 500 bp、erbB 3 644 bp、erbB 4 416 bp。这些序列与其他物种的cDNA序列高度同源。因此，这些结果表明，erbB亚家族的mRNA在犬乳腺肿瘤的表达起着重要的作用，在乳腺肿瘤的发生。
ErbB subfamily genes, known as proto-oncogenes, encode receptor tyrosine kinases, and are expressed in relation to tumorigenesis of the mammary gland in humans. In this study, we examined the expression of erbB subfamily mRNAs in two canine normal mammary glands and 12 mammary tumor samples by reverse transcriptase-coupled polymerase chain reaction (RT-PCR). Each primer set was designed from the nucleotide sequence of the region conserved in erbB subfamily cDNA among other species. No erbB subfamily mRNAs were expressed in the normal mammary gland. In contrast, all of the subfamily mRNAs were expressed in a benign mammary tumor, and more than one type of the subfamily mRNA were observed in 11 malignant mammary tumors. The length of RT-PCR products were 380 bp for erbB1, 500 bp for erbB2, 644 bp for erbB3, and 416 bp for erbB4. These sequences were highly homologous to the cDNA sequences of other species. Therefore, these results suggest that the expression of erbB subfamily mRNAs in canine mammary tumors plays an important role in tumorigenesis of the mammary gland.
影响因子：
56.9
作者：
SLAMON, DJ;CLARK, GM;MCGUIRE, WL
通讯作者：
MCGUIRE, WL
发育中的小鼠乳腺中 ERBB 受体的动态表达和激活。
DOI：
--
发表时间：
1998
期刊：
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research.
影响因子：
--
作者：
Schroeder,JA;Lee,DC
通讯作者：
Lee,DC
影响因子：
14.9
作者：
Oh, JJ;Grosshans, DR;Slamon, DJ
通讯作者：
Slamon, DJ