Pre-mRNA splicing: the discovery of a new spliceosome doubles the challenge.
Pre-mRNA splicing: the discovery of a new spliceosome doubles the challenge.
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DOI：
10.1016/s0968-0004(97)01018-9
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发表时间：
1997-04
影响因子：
13.8
通讯作者：
W. Tarn;Joan A. Steitz
中科院分区：
文献类型：
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作者：
W. Tarn;Joan A. Steitz
一类罕见的前mRNA内含子与非典型的共识序列已被确定在后生动物基因。切除这些内含子的新的低丰度剪接体含有一个与主要剪接体（U 5）共同的小核核糖核蛋白（snRNP）和四个不同于但在结构和功能上类似于U1，U2和U4-U6的snRNP。AT-AC剪接体假定核心的RNA组分的结构支持剪接体活性中心的当前模型，并提出了关于剪接体进化的诱人问题。
A rare class of pre-mRNA introns with non-canonical consensus sequences has been identified in metazoan genes. The novel, low-abundance spliceosome that excises these introns contains one small nuclear ribonucleoprotein (snRNP) in common with the major spliceosome (U5) and four snRNPs that are distinct from, but structurally and functionally analogous to U1, U2 and U4–U6. The architecture of RNA components at the presumptive core of the AT-AC spliceosome supports current models of the spliceosomal active center and raises tantalizing questions about spliceosome evolution.