Biochemical characterization of rat intestine development using high-resolution magic-angle-spinning 1H NMR spectroscopy and multivariate data analysis.
Biochemical characterization of rat intestine development using high-resolution magic-angle-spinning 1H NMR spectroscopy and multivariate data analysis.
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DOI：
10.1021/pr050032r
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发表时间：
2005-06
影响因子：
4.4
通讯作者：
Yulan Wang;Huiru Tang;E. Holmes;J. Lindon;M. Turini;N. Sprenger;G. Bergonzelli;L. Fay;S. Kochhar;J. Nicholson
中科院分区：
文献类型：
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作者：
Yulan Wang;Huiru Tang;E. Holmes;J. Lindon;M. Turini;N. Sprenger;G. Bergonzelli;L. Fay;S. Kochhar;J. Nicholson
我们报告了使用高分辨率魔角旋转（HRMAS）（1）H NMR光谱获得的小肠样品的代谢谱的细节。使用标准一维和二维（1）H NMR光谱脉冲序列，在600 MHz光谱仪上分析雄性Long Evans大鼠空肠和回肠的完整样品。回肠和空肠的代谢特征主要包括多种氨基酸、脂质、甘油磷酸胆碱（GPC）、胆碱、肌酸和乙醇、多种羧酸（包括乙酸盐和乳酸盐）以及核苷碱基（包括胞嘧啶、异胞嘧啶和尿嘧啶）。将主成分分析（PCA）应用于这些NMR数据，以表征空肠和回肠组织之间的生化差异。与回肠相比，空肠含有较高水平的脂质、GPC、胆碱、乳酸盐和肌酸酐，但较低水平的氨基酸和醋酸盐。此外，研究了幼龄大鼠（15、36天和3-4月龄）肠组织生化组成的年龄依赖性。一般而言，血脂、乳酸、牛磺酸和肌酐水平与年龄呈正相关，而氨基酸和GPC在老年组中下降。这项研究将为进一步研究评估营养、应激和肠道微生物群对肠道组成的代谢后果提供代谢参考。
We report details of metabolic profiles for small intestinal samples obtained using high-resolution magic-angle-spinning (HRMAS) (1)H NMR spectroscopy. Intact samples of jejunum and ileum from male Long Evans rats were analyzed on a 600 MHz spectrometer using standard one and two-dimensional (1)H NMR spectroscopic pulse sequences. The metabolic profiles of ileum and jejunum predominantly comprised a number of amino acids, lipids, glycerophosphocholine (GPC), choline, creatine, and ethanol, a number of carboxylic acids including acetate and lactate, and nucleoside bases including cytosine, isocytosine, and uracil. Principal component analysis (PCA) was applied to these NMR data to characterize the biochemical differences between jejunum and ileum tissues. Compared with ileum, jejunum contained higher levels of lipids, GPC, choline, lactate and creatinine, but lower levels of amino acids and acetate. In addition, the age dependence of the biochemical composition of intestinal tissues from young rats (15, 36 days and 3-4 months old) was studied. In general, levels of lipids, lactate, taurine and creatinine were positively correlated with age while amino acids and GPC decreased in the older age group. This study will provide a metabolic reference for further studies assessing the metabolic consequences of nutrition, stress and gut microbiota on intestinal composition.