Elevated serum IP-10 levels observed in type 1 diabetes
Elevated serum IP-10 levels observed in type 1 diabetes
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DOI：
10.2337/diacare.24.3.510
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发表时间：
2001-03-01
期刊：
影响因子：
16.2
通讯作者：
Narumi, S
中科院分区：
文献类型：
--
作者：
Shimada, A;Morimoto, J;Narumi, S
目的-尽管大多数1型糖尿病患者被认为患有T细胞介导的自身免疫性疾病，但在1型糖尿病病例中测量胰腺β细胞特异性T细胞功能的方法尚未建立。在这里，我们专注于干扰素诱导蛋白-10（IP-10），一种促进活化的辅助性T细胞1（Th 1）迁移的趋化因子，并测量了1型糖尿病患者的血清IP-10水平，1型糖尿病被认为是Th 1介导的疾病。并测定自身抗体（GAD和胰岛素瘤相关蛋白-2 [IA-2]）和IP-10的水平。糖尿病患者的抗体阳性或两者都被归类为Ab（+）1型，和那些都是阴性的被归类为Ab（-）1型。为了评估胰岛抗原特异性反应，使用了来自有或没有GAD刺激的患者的外周血，并进行了细胞内细胞因子染色用于流式细胞术。结果-Ab（+）和Ab（-）1型组均显示出比健康受试者显著更高的血清IP-10水平初发Ab（+）组IP-10水平显著高于长期Ab（+）组（P < 0.001和P <0.05）。此外，有一个显着的正相关关系，血清IP-10水平和GAD反应性干扰素产生的CD 4+细胞在阿比特龙1型group.CONCLUSIONS -我们的研究结果表明，血清IP-10浓度的测量是有用的1型糖尿病患者。
OBJECTIVE - Although most patients with type 1 diabetes are considered to have T-cell-mediated autoimmune disease, a method of measuring of pancreatic beta -cell-specific T-cell function in cases of type 1 diabetes has yet to be established. Here, we focused on interferon-inducible protein-10 (IP-10), a chemokine that promotes the migration of activated T-helper 1 (Th1) cells and measured serum IP-10 levels in patients with human type 1 diabetes, which is regarded as a Th1-mediated disease.RESEARCH DESIGN AND METHODS - Serum samples were obtained from diabetic patients, and the levels of autoantibodies (GAD and insulinoma-associated protein-2 [IA-2]) and IP-10 were measured. Diabetic patients positive for either or both of the autoantibodies were classified as Ab(+) type 1, and those negative for both were classified as Ab(-) type 1. To evaluate islet antigen-specific responses, peripheral blood from patients stimulated with or without GAD was used, and intracellular cytokine staining for flowcytometry was performed.RESULTS - The Ab(+) and Ab(-) type 1 groups both showed a significantly higher serum IP-10 level than the healthy subjects (P < 0.001 and P < 0.05, respectively), and the IP-10 level in the recent-onset Ab(+) subgroup was significantly higher than that in the established (longstanding) Abi subgroup (P < 0.002). Furthermore, there was a significant positive correlation between the serum IP-10 level and the number of GAD-reactive -interferon-producing CD4+ cells in the Abi type 1 group (P < 0.007).CONCLUSIONS - Our findings demonstrate that measurement of serum IP-10 concentrations is useful in patients with type 1 diabetes.