Gut microbial composition difference between pediatric ALL survivors and siblings.
Gut microbial composition difference between pediatric ALL survivors and siblings.
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DOI：
10.1080/08880018.2020.1759740
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发表时间：
2020-09
影响因子：
1.7
通讯作者：
Yang E
中科院分区：
文献类型：
--
作者：
Thomas R;Wong WSW;Saadon R;Vilboux T;Deeken J;Niederhuber J;Hourigan SK;Yang E
急性淋巴细胞白血病（ALL）是最常见的儿童癌症，治愈率高，导致长期存活者人数增加。儿童ALL的成年幸存者患肥胖、心血管疾病和其他慢性疾病的风险增加。我们假设ALL治疗与长期肠道微生物组改变相关，这些改变有助于慢性疾病的易感性。我们进行了一项初步研究，以测试是否可以检测到儿童ALL幸存者及其兄弟姐妹的粪便微生物群之间的差异。从38名19岁以下的个体中收集粪便样本，这些个体在完成ALL治疗后至少1年。从16名健康同胞收集的粪便样本作为对照。对粪便样本进行16 S核糖体RNA基因测序。将幸存者的微生物群与兄弟姐妹对照进行比较，在α或β多样性方面没有发现统计学显著差异。然而，在稀疏偏最小二乘判别分析（sPLS-DA）中来自组分1的前10个操作分类单位（OTU）中，幸存者与兄弟姐妹中具有不同的相对丰度，映射到粪杆菌属的OTU在幸存者中耗尽。在儿童ALL的儿童幸存者及其兄弟姐妹之间发现了肠道微生物组成的差异。具体而言，保护性粪杆菌在幸存者中耗尽，这让人想起在儿童ALL的成年幸存者中发现的肠道微生物群改变，并在肥胖症中报告，表明小儿ALL幸存者的微生物群改变始于儿童时期，并可能在生存后期的慢性疾病易感性中发挥作用。
Acute lymphoblastic leukemia (ALL) is the most common childhood cancer with high cure rates leading to rising numbers of long-term survivors. Adult survivors of childhood ALL are at increased risk of obesity, cardiovascular disease, and other chronic illnesses. We hypothesize that ALL therapy is associated with long-term gut microbiome alterations that contribute to predisposition to chronic medical conditions. We conducted a pilot study to test whether differences can be detected between stool microbiota of pediatric ALL survivors and their siblings. Stool samples were collected from 38 individuals under age 19 who were at least 1 year after completion of therapy for ALL. Stool samples collected from 16 healthy siblings served as controls. 16S ribosomal RNA gene sequencing was performed on the stool samples. Comparing microbiota of survivors to sibling controls, no statistically significant differences were found in alpha or beta diversity. However, among the top 10 operational taxonomic units (OTUs) from component 1 in sparse partial least squares discriminant analysis (sPLS-DA) with different relative abundance in survivors versus siblings, OTUs mapping to the genus Faecalibacterium were depleted in survivors. Differences in gut microbial composition were found between pediatric survivors of childhood ALL and their siblings. Specifically, the protective Faecalibacterium is depleted in survivors, which is reminiscent of gut microbiota alteration found in adult survivors of childhood ALL and reported in obesity, suggesting that microbiota alterations in pediatric ALL survivors start in childhood and may play a role in predisposition to chronic illness in later years of survivorship.
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影响因子：
1.2
作者：
Brown, Austin L.;Lupo, Philip J.;Kamdar, Kala Y.
通讯作者：
Kamdar, Kala Y.
DOI：
10.1016/s1470-2045(14)70265-7
发表时间：
2014-07
期刊：
The Lancet. Oncology
影响因子：
--
作者：
Essig S;Li Q;Chen Y;Hitzler J;Leisenring W;Greenberg M;Sklar C;Hudson MM;Armstrong GT;Krull KR;Neglia JP;Oeffinger KC;Robison LL;Kuehni CE;Yasui Y;Nathan PC
通讯作者：
Nathan PC
影响因子：
64.5
作者：
Cox LM;Yamanishi S;Sohn J;Alekseyenko AV;Leung JM;Cho I;Kim SG;Li H;Gao Z;Mahana D;Zárate Rodriguez JG;Rogers AB;Robine N;Loke P;Blaser MJ
通讯作者：
Blaser MJ
影响因子：
4.8
作者：
Biagi, E.;Zama, D.;Candela, M.
通讯作者：
Candela, M.
影响因子：
45.3
作者：
Hudson, Melissa M.;Oeffinger, Kevin C.;Ness, Kirsten K.
通讯作者：
Ness, Kirsten K.