[Normal pyrimidine 5'-nucleotidase activity level of Japanese subjects and a significance as a marker for low concentration of lead in blood (author's transl)].
[Normal pyrimidine 5'-nucleotidase activity level of Japanese subjects and a significance as a marker for low concentration of lead in blood (author's transl)].
复制标题
[日本受试者的正常嘧啶5-核苷酸酶活性水平及其作为血液中低浓度铅的标志物的意义（作者翻译）]。
DOI：
10.1265/jjh.36.518
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
K. Saito
中科院分区：
文献类型：
--
作者：
Y. Sato;T. Sasaki;N. Taniguchi;K. Saito
本文测定了209例健康成人红细胞嘧啶5 ′-核苷酸酶的正常活性水平。为了确定其作为低浓度铅暴露的生物学指标的价值，研究了血铅浓度与该酶活性水平之间的剂量-活性关系，以及不同血铅浓度的受试者中不同底物浓度的CMP和其他金属对嘧啶5 '-核苷酸酶的影响。以209名健康成人、109名男性和100名女性、7名铅冶炼工人和3名电池溶解厂职员为研究对象。发现受试者的嘧啶5 '-核苷酸酶活性水平的平均值和标准差为13.9±3.2（μ mol Pi/ gHb，hr）。性别、年龄差异无统计学意义，但地区差异有统计学意义。嘧啶5 ′-核苷酸酶活性的对数值与血铅浓度呈显著负相关。在体外添加试验中，10 ~（-4）M铅溶液使嘧啶5 ′-核苷酸酶活性显著降低，而锌对该酶活性无影响。上述结果提示红细胞嘧啶5 ′-核苷酸酶可能是亚临床铅暴露的一个指标。
Normal enzymic activity levels of erythrocyte pyrimidine 5'-nucleotidase were measured in 209 healthy adults. To determine its value as a biological index of the exposure to low level concentrations of lead, the dose-activity relationship between the lead concentration in the blood and the activity level of this enzyme was investigated with various substrate concentrations of CMP and the effects of other metal on pyrimidine 5'-nucleotidase in the subjects with the various concentration of lead in blood. Two hundred and nine healthy adults, 109 male and 100 female subjects, 7 lead refinery workers and 3 clerks in a battery dissolution factory were used as subjects. The mean and standard deviation of pyrimidine 5'-nucleotidase activity levels for the subjects were found to be 13.9±3.2 (μmoles Pi/ gHb, hr). No significant difference was observed for sex or age, but regional differences were significant. A remarkably high negative correlation between logarithmic values of pyrimidine 5'-nucleotidase activity and lead concentrations in the blood was observed. For the addition test in vitro, a 10-4M lead solution decreased the pyrimidine 5'-nucleotidase activity level markedly, but effects of zinc on this enzyme were not observed. The above results suggest that erythrocyte pyrimidine 5'-nucleotidase may be an index of subclinical lead exposure.