Serotonin levels in platelet-poor plasma and whole blood in people with type 2 diabetes with chronic kidney disease
Serotonin levels in platelet-poor plasma and whole blood in people with type 2 diabetes with chronic kidney disease
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DOI：
10.1016/j.diabres.2011.06.020
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发表时间：
2011-11-01
影响因子：
5.1
通讯作者：
Takahashi, Hakuo
中科院分区：
文献类型：
--
作者：
Hara, Katsuko;Hirowatari, Yuji;Takahashi, Hakuo
目的：糖尿病（DM）患者易发生动脉粥样硬化。动脉粥样硬化激活血小板;活化的血小板释放5-羟色胺，因此，血液中5-羟色胺水平的评估可能是一个有价值的生物标志物，为未来的心血管事件的风险。方法：血浆5-羟色胺水平从糖尿病合并慢性肾脏疾病的患者进行了测定，使用HPLC和健康对照组的5-羟色胺水平进行了比较。结果：糖尿病患者贫血小板血浆5-羟色胺浓度明显高于正常对照组（P < 0.01），也明显高于糖尿病患者中、重度肾功能损害组（P <0.05）。第2组患者全血5-羟色胺浓度显著低于健康对照组（P < 0.01）。结果：1、2组血浆/全血比值均显著高于健康对照组（P < 0.01）。结论：糖尿病合并轻度肾功能损害患者血小板活化释放5-羟色胺进入血浆。当肾损伤进展时，血小板过度活化以释放血清素。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Aims: Patients with diabetes mellitus (DM) are prone to atherosclerosis. Atherosclerosis activates platelets; activated platelets release serotonin, and therefore, evaluation of serotonin levels in blood could be a valuable biomarker for future risk of cardiovascular events.Methods: Plasma serotonin levels obtained from patients with DM complicated with chronic kidney disease were measured using HPLC and were compared to serotonin levels of healthy control subjects. Patients with DM were classified into 2 subgroups of mildly (group 1) and moderately/severely (group 2) impaired renal function.Results: Serotonin concentration in platelet-poor plasma for group 1 was significantly higher than that of healthy control subjects (p < 0.01), and was significantly higher than that of patients from group 2 (p < 0.05). The concentration of serotonin in whole blood for group 2 patients was significantly lower than that measured from healthy control subjects (p < 0.01). The ratio of the plasma to whole blood level was significantly elevated in both groups 1 and 2 compared with healthy controls (p < 0.01).Conclusions: Our results indicate that platelets are activated to release serotonin into plasma in diabetic patients with mildly impaired renal function. When renal damage is advanced, platelets are over-activated to release serotonin. (C) 2011 Elsevier Ireland Ltd. All rights reserved.