Equibiaxial deformation-induced injury of alveolar epithelial cells in vitro
Equibiaxial deformation-induced injury of alveolar epithelial cells in vitro
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DOI：
10.1152/ajplung.1998.275.6.l1173
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发表时间：
1998-12-01
影响因子：
4.9
通讯作者：
Margulies, SS
中科院分区：
文献类型：
--
作者：
Tschumperlin, DJ;Margulies, SS
肺泡上皮基底膜变形伴肺膨胀与呼吸机诱导肺损伤发展过程中的血气屏障破坏有关。为了确定肺泡上皮细胞对变形诱导损伤的易感性，我们开发了一种细胞拉伸装置，将细胞置于循环、等双轴应变下。原代培养的肺泡上皮II型细胞在播种后1天和5天进行检测，在此期间细胞发生了主要的形态和表型变化。细胞的表面积变化分别为12,24,37,50%，这对应于肺膨胀率分别为总肺活量的60,80,100和100%。变形诱导的肺泡上皮细胞损伤，用荧光细胞活力测定，随着变形程度的增加而增加，随着播种后时间的推移而减少。在培养1天后拉伸的细胞中，在使用的变形范围内，单次变形后的死亡细胞百分比从0.5到72%不等。在细胞拉伸5天后，当暴露于相同的变形方案时，死亡细胞的百分比从0到9%不等。这些结果表明，随着培养时间的推移，肺泡上皮细胞的形态和表型变化从根本上改变了肺泡上皮细胞对变形的易感性。
Deformation of the alveolar epithelial basement membrane with lung inflation has been implicated in blood-gas barrier breakdown during the development of ventilator-induced lung injury. To determine the vulnerability of alveolar epithelial cells to deformation-induced injury, we developed a cell-stretching device that subjects cells to cyclic, equibiaxial strains. Alveolar epithelial type II cells from primary culture were tested 1 and 5 days after seeding, during which time the cells underwent major morphological and phenotypic changes. Cells were subjected to changes in surface area of 12, 24, 37, and 50%, which corresponded to lung inflation of similar to 60, 80, 100, and >100% of total lung capacity. Deformation-induced injury of alveolar epithelial cells, assessed with a fluorescent cell viability assay, increased with deformation magnitude and decreased with time elapsed after seeding. In cells stretched after 1 day in culture, the percentage of dead cells after a single deformation ranged from 0.5 to 72% over the range of deformations used. In cells stretched at 5 days, the percentage of dead cells ranged from 0 to 9% when exposed to identical deformation protocols. These results suggest that morphological and phenotypic changes with time in culture fundamentally change the vulnerability of alveolar epithelial cells to deformation.