Opposite effects of amphetamine self-administration experience on dendritic spines in the medial and orbital prefrontal cortex
Opposite effects of amphetamine self-administration experience on dendritic spines in the medial and orbital prefrontal cortex
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DOI：
10.1093/cercor/bhh136
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发表时间：
2005-03-01
期刊：
影响因子：
3.7
通讯作者：
Robinson, TE
中科院分区：
文献类型：
--
作者：
Crombag, HS;Gorny, G;Robinson, TE
我们研究了苯丙胺自我给药经验(或蔗糖奖励训练)对伏隔核(NACC)、内侧核(MPC)和眶前额叶皮质(OFC)以及海马(CA1和Dentate)树突形态(棘密度)的长期影响。不同组的大鼠在持续强化训练的情况下接受鼻部注射安非他明(0.125 mg/kg/inf)或注射蔗糖丸，每天测试2小时，持续14-20天。最后一次训练一个月后，收集大脑并进行高尔基-考克斯染色。我们发现：(I)苯丙胺自我给药经验选择性地增加了NACC中棘神经元和MPC区锥体神经元上的脊椎密度；(Ii)相反，安非他明自我给药降低了OFC的脊椎密度，而蔗糖奖励训练增加了脊椎密度；(Iii)苯丙胺自我给药和蔗糖奖励经验都增加了CA1区的棘细胞，但对齿状回没有影响。因此，苯丙胺自我给药经验在大鼠前脑中产生了长期的和区域选择性的形态变化-这些变化可能是慢性药物暴露的一些持久的精神运动、认知和动机后果的基础。
We studied the long-term effects of amphetamine self-administration experience (or sucrose reward training) on dendritic morphology (spine density) in nucleus accumbens (Nacc), medial (MPC) and orbital prefrontal cortex (OFC), and hippocampus (CA1 and dentate). Independent groups of rats were trained under a continuous schedule of reinforcement to nose-poke for infusions of amphetamine (0.125 mg/kg/inf) or to receive sucrose pellets during 2 h daily test sessions for 14-20 days. One month after the last training session, the brains were collected and processed for Golgi-Cox staining. We found that: (i) amphetamine self-administration experience selectively increased spine density on medium spiny neurons in the Nacc and on pyramidal neurons in the MPC; (ii) in contrast, amphetamine self-administration decreased spine density in the OFC, whereas sucrose-reward training increased spine density; and (iii) both amphetamine self-administration and sucrose-reward experience increased spines in the CA1, but had no effect in the dentate gyrus. Thus, amphetamine self-administration experience produces long-lasting and regionally-selective morphological alterations in rat forebrain - alterations that may underlie some of the persistent psychomotor, cognitive and motivational consequences of chronic drug exposure.