Drosophila Casein Kinase 2 (CK2) Promotes Warts Protein to Suppress Yorkie Protein Activity for Growth Control
Drosophila Casein Kinase 2 (CK2) Promotes Warts Protein to Suppress Yorkie Protein Activity for Growth Control
复制标题
果蝇酪蛋白激酶 2 (CK2) 促进疣蛋白抑制约克蛋白活性以控制生长
DOI：
10.1074/jbc.m114.580456
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发表时间：
2014-11-28
影响因子：
4.8
通讯作者：
Zhang, Lei
中科院分区：
文献类型：
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作者：
Hu, Lianxin;Huang, Hongling;Zhang, Lei
果蝇海马信号调节Wts活性磷酸化和抑制Yki，以控制组织生长。CK 2广泛表达并参与多种信号通路。在这项研究中，我们报告说，果蝇CK 2促进Wts活性磷酸化和抑制Yki活性，这是独立的Hpo诱导的Wts促进。在体内，CK 2过表达抑制hpo突变体诱导的扩增（Ex）上调和过度生长表型，而它不能影响wts突变体。与此一致，CK 2的敲低上调Hpo途径靶表达。我们还发现，果蝇CK 2作为细胞死亡抑制剂对组织生长是必不可少的，因为在发育盘中敲低CK 2会诱导严重的生长缺陷以及半胱天冬酶3信号。综上所述，我们的研究结果揭示了CK 2的双重作用;虽然它的主要作用是促进细胞存活，但它也可能是一种潜在的生长抑制剂。
Drosophila Hippo signaling regulates Wts activity to phosphorylate and inhibit Yki in order to control tissue growth. CK2 is widely expressed and involved in a variety of signaling pathways. In this study we report that Drosophila CK2 promotes Wts activity to phosphorylate and inhibit Yki activity, which is independent of Hpo-induced Wts promotion. In vivo, CK2 overexpression suppresses hpo mutant-induced expanded (Ex) up-regulation and overgrowth phenotype, whereas it cannot affect wts mutant. Consistent with this, knockdown of CK2 up-regulates Hpo pathway target expression. We also found that Drosophila CK2 is essential for tissue growth as a cell death inhibitor as knockdown of CK2 in the developing disc induces severe growth defects as well as caspase3 signals. Taken together, our results uncover a dual role of CK2; although its major role is promoting cell survive, it may potentially be a growth inhibitor as well.