STROMAL CELLS RESPONSIBLE FOR TRANSFERRING MICROENVIRONMENT OF HEMATOPOIETIC TISSUES - CLONING INVITRO AND RETRANSPLANTATION INVIVO
STROMAL CELLS RESPONSIBLE FOR TRANSFERRING MICROENVIRONMENT OF HEMATOPOIETIC TISSUES - CLONING INVITRO AND RETRANSPLANTATION INVIVO
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DOI：
10.1097/00007890-197404000-00001
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发表时间：
1974-01-01
期刊：
影响因子：
6.2
通讯作者：
KEILISSB.IV
中科院分区：
文献类型：
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作者：
FRIEDENSTEIN, AJ;CHAILAKHYAN, RK;KEILISSB.IV
基质细胞前体通过其在单层培养物中形成成纤维细胞集落（克隆）的能力在造血细胞群体中被检测到。当接种的骨髓或脾细胞的初始密度不低于10 4个/cm 2或将照射过的骨髓细胞作为补充饲养层加入时，可达到稳定的集落形成效率。克隆形成细胞属于增殖缓慢、粘附性极强的造血组织细胞。其放射存活曲线的D 0为178 R.通过来自初始集落的细胞在体外连续传代而产生的成纤维细胞株保留形成相应造血器官的基质组织特征的能力;当成纤维细胞从骨髓培养物移植时，在肾包膜下再移植导致形成充满骨髓样细胞的骨，当成纤维细胞从脾培养物移植时，形成充满淋巴样细胞的网状组织。它的结论是，基质前体细胞负责的典型的给定同源组织的微转移。
Stromsil precursors ran bo detected in populations of homopoietic cells by their ability to form fibroblast colonies (clones) in monolayer cultures. The stable colony-forming efficiency is reached when the initial density of explanted bone marrow or spleen cells is not less than 10 4 cells/cm 2 or when irradiated bone marrow cells are added in the capacity of a supplementary feeder. The clonogenic cells belong to slowly proliferating and extremely adhesive homopoietic tissue cells. Their radiation survival curve has a D 0 of 178 R. Fibroblast strains originating by successive passages in vitro of cells from the initial colonies retain the capacity to form stromal tissue characteristic of the corresponding hemopoietic organ; retransplantation under the kidney capsule results in the formation of bone populated with myoloid cells when the fibroblasts are transplanted from bone marrow cultures, and reticular tissue populated with lymphoid cells when the fibroblasts are transplanted from spleen cultures. It is concluded that stromal precursors are the cells responsible for the transfer of the microenvironmont typical of the given homopoiotic tissue.