Effects of basic fibroblast growth factor on human gingival epithelial cells
Effects of basic fibroblast growth factor on human gingival epithelial cells
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DOI：
10.1902/jop.2002.73.12.1467
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发表时间：
2002-12-01
影响因子：
4.3
通讯作者：
Murakami, S
中科院分区：
文献类型：
--
作者：
Takayama, S;Yoshida, J;Murakami, S
背景：我们之前的报告发现碱性成纤维细胞生长因子（FGF-2；bFGF）影响牙周膜（PDL）细胞的增殖和细胞外基质的产生。在本研究中，我们检测了牙龈上皮中FGF-2的表达以及FGF-2对牙龈上皮（GE）细胞增殖反应的影响。方法：从健康牙龈上皮中分离人GE细胞，并通过逆转录聚合酶链反应（RT-PCR）检测FGF-2和FGF受体（FGFR）的mRNA表达。采用新建立的人重组FGF-2单克隆抗体，通过免疫组织学分析检测牙龈组织中FGF-2的分布，命名为BF-2。进一步，通过测量[H-3]-胸苷摄取来研究GE细胞对FGF-2的增殖反应。结果：RT-PCR显示GE细胞表达FGFR-1、FGFR-2、FGFR-3和FGFR-4 mRNA；GE细胞表达FGFR-1、FGFR-2、FGFR-3和FGFR-4 mRNA。然而，FGF-2 则不然。采用 BF-2 免疫组织化学染色，观察到 FGF-2 位于牙龈上皮的细胞间隙中，但不在上皮细胞的细胞质中。有趣的是，用肝素酶处理组织切片后，细胞间隙中 BF-2 的染色减少。此外，体外分析表明 FGF-2 增强了人类 GE 细胞的增殖反应。然而，胎牛血清共刺激抑制了FGF-2诱导的GE细胞增殖，而相同的共刺激协同增强了FGF-2诱导的PDL细胞增殖。结论：FGF-2通过乙酰肝素锚定在牙龈上皮细胞间隙中，并可能通过细胞类型调节GE细胞的生长和细胞分化。 特定受体。
Background: Our previous reports found that basic fibroblast growth factor (FGF-2; bFGF) influences the proliferation and extracellular matrix production of periodontal ligament (PDL) cells. In this study, we examined FGF-2 expression in gingival epithelium and the effect of FGF-2 on proliferative responses by gingival epithelial (GE) cells.Methods: Human GE cells were isolated from healthy gingival epithelium, and the mRNA expression of FGF-2 and FGF receptors (FGFRs) was examined by reverse transcription-polymerase chain reaction (RT-PCR). The distribution of FGF-2 in gingival tissues was detected by immunohistological analysis using the monoclonal antibody for human recombinant FGF-2, which was newly established and designated as BF-2. Further, the proliferative responses of GE cells to FGF-2 were investigated by measuring [H-3]-thymidine uptake.Results: RT-PCR revealed that GE cells express FGFR-1, FGFR-2, FGFR-3, and FGFR-4 mRNA; however, not that of FGF-2. Employing immunohistochemical staining with BF-2, FGF-2 was observed localized in the intercellular spaces of gingival epithelium, though not in the cytoplasm of epithelial cells. Interestingly, staining by BF-2 in the intercellular spaces was diminished after treatment of the tissue sections with heparitinase. Further, an in vitro analysis revealed that FGF-2 enhanced the proliferative responses of human GE cells. However, costimulation with fetal calf serum inhibited the FGF-2-induced proliferation of GE cells, whereas the same costimulation synergistically enhanced FGF-2-induced PDL cell proliferation.Conclusions: FGF-2 is anchored in the intercellular spaces of gingival epithelium via heparansulfate and may regulate the growth and cytodifferentiation of GE cells via cell-type specific receptors.