Examination of transpositional activity of nDart1 at different stages of rice development.
Examination of transpositional activity of nDart1 at different stages of rice development.
复制标题
DOI：
10.1266/ggs.86.215
复制
发表时间：
2011-06
影响因子：
1.1
通讯作者：
Mika Hayashi-Tsugane;M. Maekawa;Hirokazu Kobayashi;S. Iida;Kazuo Tsugane
中科院分区：
文献类型：
--
作者：
Mika Hayashi-Tsugane;M. Maekawa;Hirokazu Kobayashi;S. Iida;Kazuo Tsugane
作为一种阐明基因功能的有用工具，水稻转座子标记系利用了一个活跃的内源DNA转座子nDart1。为了建立高效的突变体分离系统，在水稻发育过程中评估ndar1元件的转座时间和频率是非常必要的。通过转座子显示分析，对水稻不同发育阶段新插入的检测结果进行比较，表明末节分蘖比主茎携带更多的ndar1元素。最后抽穗分蘖子代携带npart1新插入片段较多，这主要是亲本植株体细胞插入片段积累的结果。本报告表明，晚分蘖增加了产生独立突变系的可能性。
As a useful tool to elucidate gene functions, a rice transposon tagging line has been developed using an active endogenous DNA transposon, nDart1. It was highly desirable to evaluate the transposition timing and frequency of the nDart1 elements during rice development to facilitate the generation of an efficient mutant isolation system. Comparison of the detected new insertions at different stages of rice development by transposon display analysis demonstrated that the last heading tiller carry a higher number of nDart1 elements than the main culm. Moreover, it was revealed that the last heading tiller could produce progeny that carried more new insertions of nDart1 elements, mainly as a result of the accumulation of somatic insertions in the parental plant. This report demonstrates that late tillers increase the probability of producing independent mutant lines.