WATER-AVOIDANCE STRESS-INDUCED C-FOS EXPRESSION IN THE RAT-BRAIN AND STIMULATION OF FECAL OUTPUT - ROLE OF CORTICOTROPIN-RELEASING FACTOR
WATER-AVOIDANCE STRESS-INDUCED C-FOS EXPRESSION IN THE RAT-BRAIN AND STIMULATION OF FECAL OUTPUT - ROLE OF CORTICOTROPIN-RELEASING FACTOR
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DOI：
10.1016/0006-8993(94)91810-4
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发表时间：
1994-03-28
期刊：
影响因子：
2.9
通讯作者：
TACHE, Y
中科院分区：
文献类型：
--
作者：
BONAZ, B;TACHE, Y
免疫组织化学检测即刻早期基因c-fos被用来确定在暴露于已知刺激大鼠排泄量的避水应激后，大鼠大脑中神经元的活动模式。在小平台上站立避水1h可增加小球产量，并在下丘脑室旁核(PVN)、蓝斑(LC)的小细胞部、终纹床核、外侧隔核、中缝背核、A5和A1去甲肾上腺素能神经元中诱导大量Fos阳性细胞。促肾上腺皮质激素释放因子(CRF)拮抗剂，α-螺旋CRF9-41(50mug i.c.v.)水分胁迫诱导的c-fos表达减少，主要集中在PVN和LC(分别为44%和60%)，刺激排泄量减少60%。这些数据表明，水回避应激通过CRF通路激活PVN和LC神经元，从而促进结肠运动功能的刺激。
Immunohistochemical detection of the immediate-early gene c-fos was used to determine the pattern of neuronal activity in the rat brain after exposure to water-avoidance stress known to stimulate fecal output in rats. Avoidance to water for 1 h by standing on a small platform increases pellet output and induces numerous Fos-positive cells in the parvocellular part of the paraventricular nucleus of the hypothalamus (PVN), locus coeruleus (LC) and, to a lesser extent, in the bed nucleus of the stria terminalis, lateral septum, dorsal raphe nucleus and A5 and Al noradrenergic neurons. The corticotropin-releasing factor (CRF) antagonist, alpha-helical CRF9-41 (50 mug i.c.v.) reduced water-avoidance stress-induced c-fos expression mainly in the PVN and the LC (44 and 60%, respectively) and decreased by 60% the stimulated fecal output. These data indicate that water-avoidance stress activates PVN and LC neurons through CRF pathways which contribute to the stimulation of colonic motor function.