Identification and characterization of taxilin isoforms
Identification and characterization of taxilin isoforms
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DOI：
10.1016/j.bbrc.2004.05.073
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发表时间：
2004-07-02
影响因子：
3.1
通讯作者：
Shirataki, H
中科院分区：
文献类型：
--
作者：
Nogami, S;Satoh, S;Shirataki, H
突触融合蛋白家族参与胞内囊泡运输。我们最近发现taxilin，一种新的突触融合蛋白结合蛋白，它有一个长的卷曲螺旋区在其C-末端的一半。数据库搜索显示，存在两个其他分子具有长的卷曲螺旋区同源的紫杉烷在哺乳动物中。然后，我们在这里试图分离和表征这两种分子。这两种分子都与几个突触融合蛋白家族成员化学计量相互作用。然后，我们将原始的taxilin重新命名为α-taxilin，并将两种分子分别命名为β-和γ-taxilins。β-Taxilin是鸡MDP 77的人类同源物。γ-Taxilin是一种未知蛋白，北方印迹分析显示γ-Taxilin在大肠杆菌中广泛表达。β-和γ-紫杉素分别优先与syntaxin-1a和-4相互作用。taxilin家族成员与syntaxin家族成员相互作用。这些结果表明，在哺乳动物中存在至少由三个成员组成的紫杉素家族。(C)2004年爱思唯尔公司All rights reserved.
The syntaxin family is implicated in intracellular vesicle traffic. We have recently identified taxilin, a novel syntaxin-binding protein, which has a long coiled-coil region in its C-terminal half. A database search has revealed the presence of two other molecules having a long coiled-coil region homologous to that of taxilin in mammals. Then, we here attempted to isolate and characterize the two molecules. Both the two molecules stoichiometrically interacted with several syntaxin family members. Then, we renamed original taxilin alpha-taxilin and named the two molecules beta- and gamma-taxilins, respectively. beta-Taxilin was a human homologue of chicken MDP77. gamma-Taxilin was an uncharacterized protein and Northern blot analysis revealed that gamma-taxilin was ubiquitously expressed. beta- and gamma-Taxilins preferentially interacted with syntaxin-1a and -4, respectively. The taxilin family members mutually interacted with the syntaxin family members. These results indicate that there is the taxilin family composed of at least three members in mammals. (C) 2004 Elsevier Inc. All rights reserved.