Pronuclear formation of freeze-dried canine spermatozoa microinjected into mouse oocytes
Pronuclear formation of freeze-dried canine spermatozoa microinjected into mouse oocytes
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DOI：
10.1007/s10815-009-9358-y
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发表时间：
2009-10
影响因子：
3.1
通讯作者：
Hiroyuki Watanabe;Tomoyoshi Asano;Y. Abe;Y. Fukui;Hiroshi Suzuki
中科院分区：
文献类型：
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作者：
Hiroyuki Watanabe;Tomoyoshi Asano;Y. Abe;Y. Fukui;Hiroshi Suzuki
目的本研究的目的是探讨新鲜，冷冻-解冻和冷冻干燥的犬精子的体外受精能力。方法将犬精子注入小鼠卵母细胞后，卵母细胞的激活率，雄性原核形成和染色体畸变率进行了研究。冷冻干燥精子的雄性原核形成率（92.3%）显著高于新鲜精子（61.5%）和冷冻解冻精子（69.2%）（P< 0.001）。然而，在注射冷冻干燥精子的卵母细胞的染色体损伤是较高的（72.9%：P< 0.001）比新鲜（26.9%）和冻融（21.4%）spermatos.ConclusionsThese数据表明，使用小鼠卵母细胞，冷冻干燥的犬精子可能会受精犬卵母细胞，虽然染色体损伤是经常产生的。
PurposeThe aim of the present study was to investigate the fertilizing capacity of fresh, frozen-thawed and freeze-dried canine spermatozoa.MethodsAfter canine spermatozoa were injected into mouse oocytes, the rates of oocyte activation, male pronuclear formation and chromosomal aberrations were investigated.ResultsThe rates of oocyte activation were comparable (90.6–100%), no matter the sperm type injected. The percentage of male pronuclear formation was higher (P< 0.001) in the freeze-dried spermatozoa (92.3%) than the fresh (61.5%) and frozen-thawed (69.2%) spermatozoa. However, the chromosomal damage in the oocytes injected with freeze-dried spermatozoa was higher (72.9%:P< 0.001) than with fresh (26.9%) and frozen-thawed (21.4%) spermatozoa.ConclusionsThese data indicate using mouse oocytes that freeze-dried canine spermatozoa may potentially fertilize canine oocytes although chromosomal damage is frequently generated.