The tissue specificity and the genetic diversity of the alloyzymes of Solen grandis Dunker
The tissue specificity and the genetic diversity of the alloyzymes of Solen grandis Dunker
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发表时间：
2009
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通讯作者：
Chen Ai-hua
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作者：
Chen Ai-hua
采用垂直板聚丙烯酰胺凝胶电泳技术，对大竹蛏等位酶的组织特异性和绿丝的遗传多样性进行了测定（江苏省）大竹笋自然种群。六种酶（LDH、ADH、EST、MDH、ACP、SOD）（外套膜、鳃、性腺、消化腺和足肌）的酶活性进行了筛选，结果表明：结果表明：6种酶（LDH、EST、MDH和SOD）在5种组织中均具有明显的种特异性，6种酶的活性均以消化腺最高，6种酶分别由12个位点编码，其中8个位点的酶活性最高（Ldh-1、Ldh-2、Adh-1、Est-1、Mdh-1、Mdh-2、Mdh-3和Sod-2）在肌肉和消化腺中均存在多态性，占66.67%，平均观察杂合度和期望杂合度分别为0.333 3和0.373 7，平均等位基因数（Na）为2.5，有效等位基因数为1.9，Shannon信息指数为0.6242，表明吕四自然群体具有较高的遗传多样性，具有较好的遗传改良价值。
Vertical polyacrylamide gel electrophoresis was used to determine the tissue specificity of the allozymes of Solen grandis and the genetic diversity of Lvsi(Jiangsu Province) natural population of Solen grandis.Six kinds of enzymes(LDH,ADH,EST,MDH,ACP and SOD) of 5 kinds of tissues(mantle,gill,gonad,digestive gland and foot muscle) were screened.The results showed that the enzymes(LDH,EST,MDH and SOD) were obviously kinds of specific in 5 tissues.The activities of all the six kinds of enzymes were highest in digestive gland.These 6 enzymes were encoded by 12 loci,among which,8(Ldh-1,Ldh-2,Adh-1,Est-1,Mdh-1,Mdh-2,Mdh-3 and Sod-2) revealed polymorphism either in muscle or digestive gland,accounting for 66.67%.The average observed and expected heterozygosities were 0.333 3 and 0.373 7,respectively.The mean allele number(Na) and effective allele number were 2.5 and 1.9,respectively.The Shannon's information index was 0.624 2.These observations indicated that Lvsi natural population holds relatively high genetic diversity compared with others species of marine shellfishes,being valuable for genetic improvement.