Detection of amyloid-beta oligomers in human cerebrospinal fluid by flow cytometry and fluorescence resonance energy transfer.
Detection of amyloid-beta oligomers in human cerebrospinal fluid by flow cytometry and fluorescence resonance energy transfer.
复制标题
通过流式细胞术和荧光共振能量转移检测人脑脊液中的淀粉样蛋白-β寡聚物。
DOI：
--
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
G. Böhm
中科院分区：
文献类型：
--
作者：
A. N. Santos;S. Torkler;D. Nowak;Claudia Schlittig;M. Goerdes;T. Lauber;Lothar Trischmann;M. Schaupp;Monika Penz;F. Tiller;G. Böhm
阿尔茨海默病（AD）的神经病理学最近已与神经毒性淀粉样蛋白-β（Abeta）寡聚体的非纤维形式相关联，在AD患者的脑中观察到高水平的Abeta寡聚体。这表明Abeta寡聚体在AD的早期事件中发挥关键作用，强调了其早期诊断该疾病的潜力。我们已经开发了一种非常灵敏的检测方法，用于检测Abeta的寡聚体和纤维状结构，该方法基于对通过流式细胞术和荧光共振能量转移（Fret）获得的数据的多参数分析。通过分离颗粒的斑点印迹验证，该检测试剂盒可轻松检测人脑脊液（CSF）中的Abeta寡聚体。通过测量174例患有各种神经系统疾病的非痴呆对照患者的CSF样本，可以证明该方法的高度可靠性和重现性。
The neuropathology of Alzheimer's disease (AD) has been linked recently to non-fibrillar forms of neurotoxic amyloid-beta (Abeta) oligomers of which high levels are observed in the brain of AD patients. This suggests that Abeta oligomers play a key role in the early events of AD, underlining their potential for the early diagnosis of the disease. We have developed an extremely sensitive assay for the detection of oligomeric and fibrillar structures of Abeta that is based on multiparametric analysis of data obtained by flow cytometry and fluorescence resonance energy transfer (Fret). The assay readily detects Abeta oligomers in human cerebrospinal fluid (CSF) as verified by dot blot of the isolated particles. By measuring 174 CSF samples of non-demented control patients with various neurological disorders a high reliability and reproducibility of the method could be demonstrated.