Mapping quantitative trait loci for murine growth: a closer look at genetic architecture
Mapping quantitative trait loci for murine growth: a closer look at genetic architecture
复制标题
DOI：
10.1017/s0016672399004103
复制
发表时间：
1999-12-01
影响因子：
1.5
通讯作者：
Cheverud, JM
中科院分区：
文献类型：
--
作者：
Vaughn, TT;Pletscher, LS;Cheverud, JM
20多年前，D。S.法尔科纳和其他人发起了一个重要的研究途径，对小鼠体型增长的定量研究。本研究继续这一传统，定位数量性状基因座（QTL）负责小鼠的生长，如年龄特定的重量和生长期，并检查体重的遗传结构。我们在SM/J和LG/J近交系小鼠的早期F2互交（互交I）中鉴定了大量潜在的QTL。这些QTL中的许多在相同的两个株系之间的第二次F2互交（互交II）中复制。这些重复的区域为未来的精细绘图研究提供了候选区域。我们还研究了身体大小和生长QTL的组合数据集，从这两个互交，导致96个微卫星标记得分为1045个人。特定年龄的体重和生长期的遗传结构的检查导致定位20个独立的QTL，这主要是加性的性质，虽然显性被发现影响早期生长和身体大小。影响早期和晚期生长的QTL通常是不同的，映射到不同的染色体位置。正如法尔科纳所指出的，这种QTL模式表明早期和晚期小鼠生长的遗传和生理系统在很大程度上是独立的。我们还发现了性别特异性QTL的身体大小与性别二型性的进化的影响。
Over 20 years ago, D. S. Falconer and others launched an important avenue of research into the quantitative of body size growth in mice. This study continues in that tradition by locating quantitative trait loci (QTLs) responsible for murine growth, such as age-specific weights and growth periods, and examining the genetic architecture for body weight. We identified a large number of potential QTLs in an earlier F2 intercross (Intercross I) of the SM/J and LG/J inbred mouse strains. Many of these QTLs are replicated in a second F2 intercross (Intercross II) between the same two strains. These replicated regions provide candidate regions for future fine-mapping studies. We also examined body size and growth QTLs using the combined data set from these two intercrosses, resulting in 96 microsatellite markers being scored for 1045 individuals. An examination of the genetic architecture for age-specific weight and growth periods resulted in locating 20 separate QTLs, which were mainly additive in nature, although dominance was found to affect early growth and body size. QTLs affecting early and late growth were generally distinct, mapping to separate chromosome locations. This QTL pattern indicates largely separate genetic and physiological systems for early and later murine growth, as Falconer suggested. We also found sex-specific QTLs for body size with implications for the evolution of sexual dimorphism.