Persistent Infection of Simian Foamy Virus Derived from the Japanese Macaque Leads to the High-Level Expression of microRNA that Resembles the miR-1 microRNA Precursor Family
Persistent Infection of Simian Foamy Virus Derived from the Japanese Macaque Leads to the High-Level Expression of microRNA that Resembles the miR-1 microRNA Precursor Family
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DOI：
10.1264/jsme2.me19130
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发表时间：
2020-01-01
影响因子：
2.2
通讯作者：
Miyazawa, Takayuki
中科院分区：
文献类型：
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作者：
Hashimoto-Gotoh, Akira;Kitao, Koichi;Miyazawa, Takayuki
MicroRNAs （miRNAs）是一组抑制靶mrna表达的小非编码rna。miRNA的种子序列在识别靶mRNA的3'-非翻译区中起着至关重要的作用。从非洲绿猴（Chlorocebus aethiops） （SFVcae）中分离的猴泡沫病毒（SFV）感染细胞后，SFVcae长末端重复序列中编码的病毒mirna表达水平高。在本研究中，我们利用新一代测序技术研究了从日本猕猴（Macaca fuscata） （SFVmfu）分离的SFV衍生的mirna的作用和表达。结果表明，SFVmfu也表达病毒miRNAs；然而，大多数来自SFVmfu的mirna的种子序列与先前报道的来自SFVcae的mirna不同。持续感染SFVmfu的细胞强烈表达与miR-1 microRNA前体家族具有相同种子序列的miRNA。荧光素酶报告基因检测表明，该miRNA下调腺苷酸环化酶相关蛋白1的表达，而腺苷酸环化酶相关蛋白1在几种实体肿瘤中上调。目前的结果表明，SFVmfu利用病毒mirna与日本猕猴建立了长期共存关系。
MicroRNAs (miRNAs) are a group of small non-coding RNAs that suppress the expression of target mRNAs. The seed sequence of miRNA plays a crucial role in recognizing the 3'-untranslated region of the target mRNA. Cells infected with a simian foamy virus (SFV) isolated from an African green monkey (Chlorocebus aethiops) (SFVcae) showed high expression levels of viral miRNAs encoded in the long terminal repeat of SFVcae. In the present study, we investigated the roles and expression of miRNAs derived from an SFV isolated from a Japanese macaque (Macaca fuscata) (SFVmfu) using next-generation sequencing technologies. The results obtained showed that SFVmfu also expressed viral miRNAs; however, the seed sequences of most miRNAs derived from SFVmfu differed from those reported previously from SFVcae. Cells persistently infected with SFVmfu strongly expressed an miRNA with the same seed sequence as the miR-1 microRNA precursor family. Luciferase reporter assays indicated that this miRNA down-regulates the expression of adenylyl cyclase-associated protein 1, which is up-regulated in several solid tumors. The present results suggest that SFVmfu utilizes viral miRNAs to establish long-term co-existence with the Japanese macaque.