2 GENES PRESENT ON A TRANSPOSON-LIKE STRUCTURE IN LACTOCOCCUS-LACTIS ARE INVOLVED IN A CLP-FAMILY PROTEOLYTIC ACTIVITY
2 GENES PRESENT ON A TRANSPOSON-LIKE STRUCTURE IN LACTOCOCCUS-LACTIS ARE INVOLVED IN A CLP-FAMILY PROTEOLYTIC ACTIVITY
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DOI：
10.1111/j.1365-2958.1993.tb01187.x
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发表时间：
1993-03-01
影响因子：
3.6
通讯作者：
NOVEL, M
中科院分区：
文献类型：
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作者：
HUANG, DC;HUANG, XF;NOVEL, M
乳酸乳球菌亚种的乳糖蛋白酶表达载体pUCL22。乳酸杆菌CNRZ270菌株含有两个IS1076的倒置拷贝，位于3.7kb的区域两侧。对该内部区域进行了测序，发现含有两个方向相反的大的开放阅读框ORF1和ORFP。ORF1全长2289bp，推导的763个氨基酸序列与ClpA家族的ATPase相似。它包含两个保守的共识ATP结合位点。它被命名为CLPL。ORFP全长930个核苷酸，编码310个氨基酸的蛋白质。在OrFP的GenBank数据中没有发现与任何已知蛋白质的相似性。在表达CLPL和ORFP基因的大肠杆菌细胞中，检测到依赖于ATP的蛋白水解物活性增加。
The lactose-protease plasmid pUCL22 of Lactococcus lactis subsp. lactis strain CNRZ270 contained two inverted copies of IS1076 flanking a region of 3.7 kb. This internal region was sequenced and found to contain two large open reading frames, ORF1 and ORFP in opposite orientations. ORF1 consists of 2289 bp; the deduced 763-amino-acid sequence is similar to the ATPases of the ClpA family. It contains two well-conserved consensus ATP-binding sites. It was named ClpL. ORFP consists of 930 bp encoding a protein of 310 amino acids. No similarity with any known protein was found in GenBank data for ORFP. Increased ATP-dependent proteolytic activity was detected in extracts from Escherichia coli cells expressing the clpL and ORFP genes.