Fertility behaviour of rice (Oryza sativa) lines with dominant male sterile gene and inheritance of sterility and fertility restoration
Fertility behaviour of rice (Oryza sativa) lines with dominant male sterile gene and inheritance of sterility and fertility restoration
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DOI：
10.1016/j.fcr.2005.12.004
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发表时间：
2006-07
影响因子：
5.8
通讯作者：
Hao-hua He;X. Peng;Huiming Gong;Changlan Zhu;G. Ye
中科院分区：
文献类型：
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作者：
Hao-hua He;X. Peng;Huiming Gong;Changlan Zhu;G. Ye
以萍乡雄性不育水稻(PMSR)与11个可育系建立的后代群体为材料，研究了其雄性不育和育性恢复的遗传基础。结果表明，雄性不育是由两个互作(上位性)核基因决定的，一个控制不育，一个控制恢复。显性不育基因单独存在时表现为雄性不育，但与恢复基因共存时表现为正常可育。只有恢复基因的个体是正常的和可育的。研究了利用PMSR通过重复自交育成的纯合子不育系在控制和田间条件下的育性表现。雄性育性受温度和光周期的共同影响，温度对雄性育性的影响更大。诱导育性的临界温度为27-28℃。超过这一临界温度，植株仍保持不育，但在较高温度下部分可育。对温度敏感的穗部发育阶段是从二次枝原基分化(S3)到花粉母细胞减数分裂(S6)。在这些敏感时期持续高温(>；30°C)是维持男性生育能力所必需的。长光周期(15h)即使在温度下也能诱导部分育性，可能导致不育。在实践中，这条线可以被认为是热敏的。在亚热带地区，该品系完全不育期长达4周以上，适合用两系杂交制种(一对纯不育可育系)。利用低温短光周期来表现雄性不育的不育系，如来源于PMSR的雄性不育系，在任何作物中都未见报道。该品系也是第一个显性核不育系，可利用两系法制种。
The genetic basis of male sterility and fertility restoration of the Pingxiang male-sterile rice (PMSR) was studied using progeny populations created between PMSR and 11 fertile lines. It was found that the male sterility was determined by two interacting (epistatic) dominant nuclear genes, one for sterility and one for fertility restoration. The dominant sterile gene expresses as male-sterility when existing solely, but as normally fertile when coexisting with the restoration gene. The individuals with only the restoration gene are normal and fertile. A homozygous sterile line developed using PMSR by repeated selfing was characterised for its fertility behaviour under controlled and field conditions. Male fertility was affected by both temperature and photoperiod with temperature being more important. The critical temperature for inducing fertility was 27–28°C. Blow this critical temperature, plants remained sterile, but become partially fertile at higher temperature. The panicle development stages that are sensitive to temperature were from differentiation of the secondary branch primordium (S3) to the meiotic division of the pollen mother cells (S6). Continuous high temperature (>30°C) during these sensitive stages is necessary to maintain male fertility. Long photoperiod (15h) induced partial fertility even under temperature, which could induce sterility. In practice, this line can be regarded as thermo-sensitive. In the sub-tropical zone, this line has complete sterile phase longer than 4 weeks and thus is suitable for hybrid production using the two-line system (a pair of pure sterile and fertile lines). Male sterile line required low temperature and short photoperiod to express male sterility, like the one derived from PMSR, has never been reported in any crop species. This line is also the first dominant nuclear male-sterile line that could be exploited for hybrid seed production using the two-line system.