Timed action of the gene products required for septum formation in the cell cycle of Bacillus subtilis
Timed action of the gene products required for septum formation in the cell cycle of Bacillus subtilis
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枯草芽孢杆菌细胞周期中隔膜形成所需的基因产物的定时作用
DOI：
10.1128/jb.149.2.673-680.1982
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发表时间：
1982
影响因子：
3.2
通讯作者：
Y. Maruyama
中科院分区：
文献类型：
--
作者：
Y. Miyakawa;T. Komano;Y. Maruyama
用4株温度敏感的无隔膜突变体的同基因菌株研究了枯草芽孢杆菌细胞周期中隔膜形成起始所需的基因产物的作用时间。向上移位的实验，其中每个突变体的同步培养物的部分被转移到非允许的温度，表明的过渡点，在该点，细胞达到分裂的能力在非允许的温度在细胞周期中，是应变特定的。此外，热休克实验中，同步培养物的部分在转变点之前接受非许可温度一段固定时间，然后转移回许可温度，表明转移回和随后的细胞分裂之间的时间间隔是每个菌株特有的，但与热休克的年龄无关。这些结果使我们产生了这样的想法，即在B。枯草芽孢杆菌需要四种基因产物的定时作用，每种基因产物在细胞周期的特定阶段起作用。此外，DNA延伸突变体MK-526（也是隔膜缺陷型）的结果支持了我们先前的发现，即隔膜形成的起始需要先前细胞周期中DNA复制的终止。
Four isogenic strains of temperature-sensitive septationless mutants, whose mutations are located on different genes, were used to study the periods of action of the gene products required for the initiation of septum formation during the cell cycle of Bacillus subtilis. The shift-up experiments, in which portions of a synchronous culture of each mutant were transferred to the nonpermissive temperature, showed that the transition point, at which cells attained the ability to divide at the nonpermissive temperature in the cell cycle, was strain specific. Furthermore, the heat shock experiments, in which portions of a synchronous culture were subjected to the nonpermissive temperature before the transition point for a fixed period and shifted back to the permissive temperature, showed that the time interval between the shift-back and the subsequent cell division was specific to each strain but was independent of the age of heat shock. These results led us to the idea that the initiation of septum formation in B. subtilis requires the timed action of the four gene products, each of which functions at a specific stage in the cell cycle. In addition, the result with DNA elongation mutant MK-526, which is also septation defective, supported our previous findings that the initiation of septum formation requires the termination of DNA replication in the previous cell cycle.