Effects of tyrosol and farnesol on Candida albicans biofilm
Effects of tyrosol and farnesol on Candida albicans biofilm
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DOI：
10.3892/mmr.2019.9981
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发表时间：
2019-04-01
影响因子：
3.4
通讯作者：
Courtois, Philippe
中科院分区：
文献类型：
--
作者：
Sebaa, Sarra;Boucherit-Otmani, Zahia;Courtois, Philippe
The present in vitro study examined the effects of the quorum-sensing molecules farnesol and tyrosol on the development of Candida albicans biofilm in order to elucidate their role as novel adjuvants in oral hygiene. The investigation was conducted in C. albicans ATCC 10231 and C. albicans isolates from dentures and was performed in flat-bottomed 96-well polystyrene plates. Yeast growth and their capacity to form biofilms were evaluated following 24 and 48 h incubations at 37 degrees C in Sabouraud broth supplemented with 0.001-3 mM farnesol and/or 1-20 mM tyrosol. Yeast growth was assessed by turbidimetry and biofilms were quantitated by crystal violet staining, under aerobic and anaerobic conditions. The viability of the fungal cells was controlled by the culture of planktonic cells and by examination of the biofilms using fluorescence microscopy following staining with fluorescein diacetate and ethidium bromide. Farnesol at 3 mM exerted a stronger action when added at the beginning of biofilm formation (>50% inhibition) than when added to preformed biofilms (80% inhibition) than on preformed biofilms (
