Variations in the cell wall composition of maize brown midrib mutants
Variations in the cell wall composition of maize brown midrib mutants
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DOI：
10.1021/jf0260592
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发表时间：
2003-02-26
影响因子：
6.1
通讯作者：
Hatfield, RD
中科院分区：
文献类型：
--
作者：
Marita, JM;Vermerris, W;Hatfield, RD
到目前为止，发表的大多数研究都是关于玉米(Zea Mays L.)的四个棕色中脉(Bm)突变体(bm1、bm2、bm3和brn4)的。已经集中在一个或两个个体突变上，由于遗传背景、成熟度和组织来源的差异，研究之间的比较一直很困难。对四个Bm单突变体和一个bm1-bm2双突变体在相同遗传背景下(近交系A619)的茎的详细分析表明，与野生型近交系相比，它们分离的细胞壁和木质素的结构和成分发生了变化。2D-核磁共振显示BM3分离的木质素中存在大量的苯并二恶烷单元。1DC-13核磁共振显示，与野生型近交系相比，bm1和bm1-bm2突变体的乙醛水平增加。Bm3和bm1-bm2突变体的细胞壁中含有较少的Klason木质素。Bm1、bm3和bm1-bm2突变体的酯化p-香豆酸含量减少了50%，而bm3突变体中的阿魏酸水平显著升高。Bm突变株在纤维素酶处理过程中对香豆酸-木质素复合体的溶解度也存在差异，这表明Bm突变株在木质素的结构组成上也可能存在差异。
Most studies published thus far on the four brown midrib (bm) mutants (bm1, bm2, bm3, and brn4) in maize (Zea mays L.) have focused on one or two individual mutants, and comparisons between studies have been difficult because of variation in genetic backgrounds, maturity, and source of tissue. Detailed analyses of the stalks of the four bm single mutants and a bm1-bm2 double mutant in a common genetic background (inbred A619) revealed structural and compositional changes in their isolated cell walls and lignins compared to the wild-type inbred. 2D-NMR revealed a significant presence of benzodioxane units in the bm3 isolated lignin. 1D C-13 NMR revealed increased aldehyde levels in the bm1 and bm1-bm2 mutants compared to the wild-type inbred. The bm3 and bm1-bm2 mutants contained less Klason lignin in the isolated cell walls. The bm1, bm3, and bm1-bm2 mutants contained similar to50% less esterified p-coumaric acid with noticeably elevated levels of ferulate in the bm3 mutant. A difference among bm mutants in the solubility of p-coumaric acid-lignin complexes during cellulase enzyme treatment was also discovered, suggesting that the bm mutations might also differ in the structural organization of lignin.