The Preparation of Sugar Polymer-Coated Nanocapsules by the Layer-by-Layer Deposition on the Liposome
The Preparation of Sugar Polymer-Coated Nanocapsules by the Layer-by-Layer Deposition on the Liposome
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DOI：
10.1021/la9008834
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发表时间：
2009-09-01
期刊：
影响因子：
3.9
通讯作者：
Fujimoto, Keiji
中科院分区：
文献类型：
--
作者：
Fukui, Yuuka;Fujimoto, Keiji
我们打算将脂质体制备和逐层沉积结合联合收割机制备纳米胶囊。沉积壳聚糖（CHI）以在带负电荷的脂质体表面上形成阳离子聚合物层，并使用阴离子聚合物硫酸葡聚糖（DXS）或脱氧核糖核酸（DNA）进行进一步沉积。在每次沉积时，纳米胶囊的ζ-电位在正电荷和负电荷之间变化。FE-TEM显示，即使在逐层（LbL）沉积后，脂质体仍保持球形。与裸脂质体相比，胶囊壁显示其对表面活性剂Triton X-100的稳定性显著增加，并且稳定性可通过聚合物的吸附量来控制。这些表明，聚合物多层产生的脂质体表面的多糖的层层沉积。三种不同电荷的化学物质，1-羟基芘-3，6，8-三磺酸（HPTS），阿仑膦酸钠，和葡萄糖，被封装到纳米胶囊和释放被抑制的聚合物胶囊壁的电荷无关。通过将温度从25 ° C升高到60 ° C，从DNA沉积的纳米胶囊（liponano-CHI-DNA）的释放明显增加。这表明温度依赖性释放是通过将DNA变性作为温度依赖性“开关”来实现的，其影响囊壁的渗透性。
We intended to combine the liposomal preparation and the layer-by-layer deposition to prepare a nanosized capsule. Chitosan (CHI) was deposited to form the cationic polymeric layer onto a negatively charged liposomal surface and further deposition was carried out using anionic polymers dextran sulfate (DXS) or deoxyribonucleic acid (DNA). zeta-Potentials of nanocapsules changed between positive and negative charges at each deposition. FE-TEM revealed that the liposome remained a spherical shape even after the layer-by-layer (LbL) deposition. The capsule wall showed it dramatic increase in stability against the surfactant Triton X-100 compared to a bare liposome, and the stability was controllable by the adsorption amount of the polymer. These suggest that the polymer multilayer was generated on the liposome surface by the layer-by-layer depositions of polysaccharides. The three kinds of chemical substances with different charges, I-hydroxy pyrene-3;6,8-trisulfonic acid (HPTS), alendronate, and glucose, were encapsulated into nanocapsules and the release was suppressed by the polymeric capsule wall irrespective of charges. The release from DNA-deposited nanocapsules (liponano-CHI-DNA) was clearly increased by raising temperature from 25 to 60 degrees C. This indicates that the temperature-dependent release was achieved by applying DNA denaturation as a temperature-dependent "switch", which influenced the permeability of the capsule wall.