Multiple interactions between BRG1 and MHC class II promoter binding proteins.
Multiple interactions between BRG1 and MHC class II promoter binding proteins.
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BRG1 和 MHC II 类启动子结合蛋白之间的多重相互作用。
DOI：
10.1016/j.molimm.2004.09.021
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发表时间：
2005
影响因子：
3.6
通讯作者：
Fontes,JosephD
中科院分区：
文献类型：
--
作者：
Mudhasani,Rajini;Fontes,JosephD
II类反激活因子（CIITA）与染色质重塑因子brahma相关基因1 （BRG1）相互作用，是人类主要组织相容性复合体（MHC） II类基因转录激活的必要组成部分。我们在这里报道，RFXAP， dna结合RFX复合体的一个亚基，也结合BRG1，因此提供了一种在没有CIITA的情况下MHC II类基因染色质重塑的机制。RFXAP和CIITA结合在BRG1的不同区域。RFXAP蛋白结合BRG1的区域与先前报道的介导MHC II类基因转录所必需的RFXAP的最低要求片段一致。对于CIITA，我们发现BRG1与n端酸性氨基酸丰富的转激活域和含有gtp结合基序的CIITA中心区域相互作用。对缺乏CIITA或RFXAP的细胞系中MHC II类基因染色质结构的分析表明，RFXAP - brg1相互作用可能在某些细胞类型中产生染色质重塑。
The class II transactivator (CIITA) interacts with the chromatin remodeling factor brahma related gene 1 (BRG1) as a necessary component of the transcriptional activation of human major histocompatibility complex (MHC) class II genes. We report here that RFXAP, a subunit of the DNA-binding RFX complex, also binds BRG1 and therefore provides a mechanism by which MHC class II gene chromatin can be remodeled in the absence of CIITA. RFXAP and CIITA bind to different regions of BRG1. The region of the RFXAP protein that binds BRG1 coincides with the minimally required fragment of RFXAP previously reported to be necessary to mediate MHC class II gene transcription. For CIITA, we found that BRG1 interacts with both the N-terminal acidic amino acid rich transactivation domain and a central region of CIITA that contains GTP-binding motifs. Analysis of chromatin structure of MHC class II genes in cell lines lacking CIITA or RFXAP, suggests that the RFXAP–BRG1 interaction may, in some cell types, produce chromatin remodeling.