The fabrication of double-layered chitosan/gelatin/genipin nanosphere coating for sequential and controlled release of therapeutic proteins
The fabrication of double-layered chitosan/gelatin/genipin nanosphere coating for sequential and controlled release of therapeutic proteins
复制标题
双层壳聚糖/明胶/京尼平纳米球涂层的制备，用于治疗性蛋白质的顺序和受控释放
DOI：
10.1088/1758-5090/aa70c3
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发表时间：
2017-06-01
期刊：
影响因子：
9
通讯作者：
Jiang, Tao
中科院分区：
文献类型：
--
作者：
Chen, Ruiying;Cai, Xinjie;Jiang, Tao
骨再生是一个复杂的过程，包括在多种生长因子的调节下协调细胞作用的许多不同和连续的阶段。因此，其中生长因子可以按照与骨组织愈合过程一致的程序顺序掺入和释放的骨移植材料可以导致期望的临床结果。采用逐层电泳沉积法和京尼平交联法制备了双层壳聚糖/明胶/京尼平（d-CSG/G）纳米微球包衣。研究了涂层的表面形貌、物理化学性能和力学性能。细胞色素C被用作治疗模型蛋白，并成功地装载在涂层的内层和外层上。蛋白质的释放可以通过加载位置、京尼平浓度和外层厚度来控制。此外，评价了细胞对涂层的响应。实时聚合酶链反应，免疫荧光染色和细胞外基质矿化测定证实，负载的生长因子的功能完全保留后制造。总体而言，d-CSG/G纳米球涂层可能是一种有前途的生长因子递送系统，以促进骨组织再生。
Bone regeneration is a complicated process and includes a number of distinct and sequential stages of coordinated cellular actions under the regulation of multiple growth factors. Therefore, bone grafting materials in which growth factors can be incorporated and released in a programmed order in line with the bone tissue healing process may lead to desirable clinical outcomes. In the present study, a double-layered chitosan/gelatin/genipin (d-CSG/G) nanosphere coating is developed by using layer-by-layer electrophoretic deposition and genipin crosslinking. The surface morphology, physicochemical and mechanical properties of the coatings are explored. Cytochrome C is used as a therapeutic model protein and is successfully loaded on the inner and outer layers of the coating. The protein release can be controlled by the loading position, genipin concentration and thickness of the outer layer. Furthermore, the cell response to the coatings was evaluated. Real-time polymerase chain reactions, immunofluorescence staining and extracellular matrix mineralization assay confirmed that the functions of the loaded growth factor are fully preserved after fabrication. Overall, the d-CSG/G nanosphere coating could be a promising growth factor delivery system to promote bone tissue regeneration.