Identification of a syndecan 4 pseudogene.
Identification of a syndecan 4 pseudogene.
复制标题
Syndecan 4 假基因的鉴定。
DOI：
10.1080/1042517021000019296
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Terman,BruceI
中科院分区：
文献类型：
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作者：
Spring,SimoneC;Terman,BruceI
Syndecan家族的硫酸乙酰肝素蛋白多糖通过与生长因子、细胞外基质和其他分子相互作用参与细胞激活。该家族由四种蛋白质组成，它们在胞质结构域中具有相同的序列同源性。在此，我们报道了人类染色体22q12.2的5.8kb区域包含多个片段，这些片段与Syndecan 4转录本有超过80%的序列同源性，包括与Syndecan 4编码区的443个核苷酸的同源性。三项证据表明，22号染色体序列为Syndecan 4假基因。首先，需要在22号染色体序列中插入单核苷酸间隙，以保持与Syndecan 4编码序列的最大比对，这将终止密码子引入到推导的氨基酸序列中。第二，与互补的Syndecan 4序列的基因组组织相比，包含同源序列的22号染色体的总长度被压缩。第三，5.8kb的22号染色体序列含有多个Alu等重复序列，这是假基因的特征。RT-PCR和核糖核酸酶保护实验均表明Syndecan 4假基因在人脐静脉内皮细胞中转录。
The syndecan family of heparan sulfate proteoglycans participates in cellular activation through interactions with growth factors, extracellular matrix, and other molecules. The family consists of four proteins that share sequence homology within their cytosolic domains. Here we report that a 5.8 kb region of human chromosome 22q12.2 contains multiple segments that share greater than 80% sequence homology to the syndecan 4 transcript, including homology to 443 nucleotides of the syndecan 4 coding region. Three pieces of evidence indicate that the chromosome 22 sequences are a syndecan 4 pseudogene. First, single nucleotide gaps need to be inserted into the chromosome 22 sequence in order to maintain maximal alignment to the syndecan 4 coding sequence, and this introduces stop codons into a deduced amino acid sequence. Second, the total length of chromosome 22 containing the homologous sequences is compressed when compared to the genomic organization of the complementary syndecan 4 sequences. Third, the 5.8 kb chromosome 22 sequence contains multiple Alu and other repetitive sequences, and this is a property of pseudogenes. Both RT-PCR and RNase protection assays indicated that the syndecan 4 pseudogene is transcribed in human umbilical vein endothelial cells.