Expressed sequence tags of medaka (Oryzias latipes) liver mRNA.
Expressed sequence tags of medaka (Oryzias latipes) liver mRNA.
复制标题
青鳉 (Oryzias latipes) 肝脏 mRNA 的表达序列标签。
DOI：
--
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
T. Aoki
中科院分区：
文献类型：
--
作者：
I. Hirono;T. Aoki
在 lambda ZAPII 中构建了青鳉肝脏 cDNA 文库。该文库中的克隆数量约为 5 x 10(6)。随机选择了 361 个克隆，并对其中 33 个超过 1 kb 的克隆进行了测序。将这些序列与 GenBank 和 dbEST (Re.96.0) 进行比较。这 33 个克隆中的 25 个克隆编码 18 个不同的基因和 2 个不同的表达序列标签 (EST)。 18 个克隆中有 10 个的序列此前从未在鱼类中报道过。青鳉基因的密码子使用与非洲爪蟾、小鼠和人类基因的密码子使用相似，但与酵母和大肠杆菌基因的密码子使用不同。
A medaka liver cDNA library was constructed in lambda ZAPII. The number of clones in this library is approximately 5 x 10(6). Three hundred sixty-one clones were randomly selected and, of these, 33 clones with over 1 kb were sequenced. These sequences were compared with GenBank and the dbEST (Re.96.0). Twenty-five clones of these 33 clones encoded 18 different genes and 2 different expressed sequence tags (ESTs). Sequences of 10 of the 18 clones had not previously been reported in fish. The codon usage of medaka genes is similar to that of Xenopus, mouse, and human genes, but not similar to that of yeast and Escherichia coli genes.