Attenuated chromatin compartmentalization in meiosis and its maturation in sperm development
Attenuated chromatin compartmentalization in meiosis and its maturation in sperm development
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DOI：
10.1038/s41594-019-0189-y
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发表时间：
2019-03-01
影响因子：
16.8
通讯作者：
Namekawa, Satoshi H.
中科院分区：
文献类型：
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作者：
Alavattam, Kris G.;Maezawa, So;Namekawa, Satoshi H.
生殖细胞表现出独特的基因表达程序，并在受精卵中恢复全能性。在这里，我们进行Hi-C分析，以检查在精子发生过程中的男性生殖细胞的3D染色质组织。我们发现，高度分隔的3D染色质组织特征的间期核在减数分裂前期衰减。减数分裂前期主要是由短程染色体内相互作用，代表一个浓缩的形式类似于有丝分裂染色体。然而，与有丝分裂染色体不同，减数分裂染色体在前期显示出弱的基因组区室化、弱的拓扑关联结构域和局部点相互作用。在减数分裂后的圆形精子细胞中，基因组区室化增加，并产生在成熟精子中观察到的强烈区室化。在减数分裂性染色体失活过程中，X染色体缺乏结构域。我们认为，男性减数分裂发生在全球重新编程的3D染色质组织和染色质区室化的加强发生在精子发生，准备下一代的生活。
Germ cells manifest a unique gene expression program and regain totipotency in the zygote. Here, we perform Hi-C analysis to examine 3D chromatin organization in male germ cells during spermatogenesis. We show that the highly compartmentalized 3D chromatin organization characteristic of interphase nuclei is attenuated in meiotic prophase. Meiotic prophase is predominated by short-range intrachromosomal interactions that represent a condensed form akin to that of mitotic chromosomes. However, unlike mitotic chromosomes, meiotic chromosomes display weak genomic compartmentalization, weak topologically associating domains, and localized point interactions in prophase. In postmeiotic round spermatids, genomic compartmentalization increases and gives rise to the strong compartmentalization seen in mature sperm. The X chromosome lacks domain organization during meiotic sex-chromosome inactivation. We propose that male meiosis occurs amid global reprogramming of 3D chromatin organization and that strengthening of chromatin compartmentalization takes place in spermiogenesis to prepare the next generation of life.