Evolutionary and functional analysis reveals the crucial roles of receptor-like proteins in resistance to Valsa canker in Rosaceae
Evolutionary and functional analysis reveals the crucial roles of receptor-like proteins in resistance to Valsa canker in Rosaceae
复制标题
进化和功能分析揭示了蔷薇科类受体蛋白在抵抗瓦尔萨溃疡病中的关键作用
DOI：
10.1093/jxb/erac417
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发表时间：
2022
影响因子：
6.9
通讯作者：
Cunwu Zuo
中科院分区：
文献类型：
--
作者：
Hongqiang Yu;E Sun;Xia Mao;Zhongjian Chen;Tong Xu;Longgang Zuo;Daji Jiang;Yanan Cao;Cunwu Zuo
抽象的。蔷薇科植物是一种重要的经济植物，可受多种病原微生物的影响，其中一些病原微生物可造成严重的生产损失。作为一种模式识别受体，类受体蛋白(RLP)被认为是植物免疫的重要调节因子。在全基因组鉴定、生物信息学分析和功能测定的基础上，我们研究了RLP的进化特征，特别是那些调控腐烂病的RLP，这是一种影响苹果和梨生产的毁灭性真菌疾病。从包括9个蔷薇科植物在内的19个种的基因组中共获得3028个RLP，分为24个亚科。抗病梨砧木‘Duli-G03’对腐烂病信号的反应涉及5个亚家族和7个共表达模块。随后筛选出14个RLP作为抗性调控的候选基因。其中，PbeRP23(Chr13.g24394)和PbeRP27(Chr16.g31400)被鉴定为能迅速提高‘Duli-G03’抗性并引发免疫应答的关键抗病基因，因此它们在抗腐烂病的功能开发和育种应用上具有潜力。此外，作为这项工作的结果，我们建立了抗病RLP的分类和筛选的最佳方法。
Abstract. Rosaceae is an economically important plant family that can be affected by a multitude of pathogenic microbes, some of which can cause dramatic losses in production. As a type of pattern-recognition receptor, receptor-like proteins (RLPs) are considered vital regulators of plant immunity. Based on genome-wide identification, bioinformatic analysis, and functional determination, we investigated the evolutionary characteristics of RLPs, and specifically those that regulate Valsa canker, a devastating fungal disease affecting apple and pear production. A total of 3028 RLPs from the genomes of 19 species, including nine Rosaceae, were divided into 24 subfamilies. Five subfamilies and seven co-expression modules were found to be involved in the responses to Valsa canker signals of the resistant pear rootstock Pyrus betulifolia ‘Duli-G03’. Fourteen RLPs were subsequently screened as candidate genes for regulation of resistance. Among these, PbeRP23 (Chr13.g24394) and PbeRP27 (Chr16.g31400) were identified as key resistance genes that rapidly enhance the resistance of ‘Duli-G03’ and strongly initiate immune responses, and hence they have potential for further functional exploration and breeding applications for resistance to Valsa canker. In addition, as a consequence of this work we have established optimal methods for the classification and screening of disease-resistant RLPs.