Laser ablation reveals the impact of Cdc15p on the stiffness of the contractile ring.
Laser ablation reveals the impact of Cdc15p on the stiffness of the contractile ring.
复制标题
激光烧蚀揭示了CdC15P对收缩环硬度的影响。
DOI：
10.1091/mbc.e21-10-0515
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发表时间：
2022-05-15
影响因子：
3.3
通讯作者：
Laplante, Caroline
中科院分区：
文献类型：
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作者：
Moshtohry, Mohamed;Bellingham-Johnstun, Kimberly;Elting, Mary Williard;Laplante, Caroline
控制收缩环收缩的机制仍然知之甚少，但对于理解驱动胞质分裂的力至关重要。我们使用激光消融裂变酵母细胞解开这些力学重点Cdc15 p作为一个假定的锚定蛋白的作用。我们的工作表明，被切断的收缩环弹回到一个有限的点，留下一个间隙，如果小于0.1微米，就可以愈合。在Cdc15p耗尽的细胞中，切断的收缩环表现出夸张的反冲，这表明反冲是有限的锚定环的质膜。基于切断的收缩环的物理模型，我们提出Cdc15p影响的收缩环的刚度比粘性阻力。
The mechanics that govern the constriction of the contractile ring remain poorly understood yet are critical to understanding the forces that drive cytokinesis. We used laser ablation in fission yeast cells to unravel these mechanics focusing on the role of Cdc15p as a putative anchoring protein. Our work shows that the severed constricting contractile ring recoils to a finite point leaving a gap that can heal if less than ∼1 µm. Severed contractile rings in Cdc15p-depleted cells exhibit an exaggerated recoil, which suggests that the recoil is limited by the anchoring of the ring to the plasma membrane. Based on a physical model of the severed contractile ring, we propose that Cdc15p impacts the stiffness of the contractile ring more than the viscous drag.