Isolation and characterization of lymphocyte-like cells from a lamprey
Isolation and characterization of lymphocyte-like cells from a lamprey
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DOI：
10.1073/pnas.212527499
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发表时间：
2002-10-29
影响因子：
11.1
通讯作者：
Cooper, MD
中科院分区：
文献类型：
--
作者：
Mayer, WE;Uinuk-ool, T;Cooper, MD
在光散射条件下，用流式细胞术分离出七鳃鳗肠道淋巴细胞样细胞，用于纯化小鼠肠道淋巴细胞。纯化的七鳃鳗细胞在形态上与哺乳动物淋巴细胞难以区分。从七鳃鳗淋巴细胞样细胞制备cDNA文库，并对8，000多个随机选择的克隆进行测序。同源性搜索比较这些EST与存放在数据库中的序列，导致许多基因同源的哺乳动物淋巴细胞，其中包括基因控制淋巴细胞生成，细胞内信号，增殖，迁移，参与先天免疫反应的淋巴细胞中主要或特征性表达的鉴定。基因密切相关的那些grathostomes控制抗原加工和运输的抗原肽可以确定，虽然没有序列具有显着的相似性的MHC，T细胞受体，或IG genes.The数据表明，淋巴细胞在七鳃鳗的进化已经达到了一个阶段，准备出现适应性免疫。
Lymphocyte-like cells in the intestine of the sea lamprey, Petromyzon marinus, were isolated by flow cytometry under light-scatter conditions used for the purification of mouse intestinal lymphocytes. The purified lamprey cells were morphologically indistinguishable from mammalian lymphocytes. A cDNA library was prepared from the lamprey lymphocyte-like cells, and more than 8,000 randomly selected clones were sequenced. Homology searches comparing these ESTs with sequences deposited in the databases led to the identification of numerous genes homologous to those predominantly or characteristically expressed in mammalian lymphocytes, which included genes controlling lymphopoiesis, intracellular signaling, proliferation, migration, and involvement of lymphocytes in innate immune responses. Genes closely related to those that in grathostomes control antigen processing and transport of antigenic peptides could be ascertained, although no sequences with significant similarity to MHC, T cell receptor, or Ig genes were found. The data suggest that the evolution of lymphocytes in the lamprey has reached a stage poised for the emergence of adaptive immunity.