Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer
Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer
复制标题
使用基于表面增强拉曼散射的免疫分析同时检测双重前列腺特异性抗原，以准确诊断前列腺癌
DOI：
10.1021/acsnano.7b01536
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发表时间：
2017-05-01
期刊：
影响因子：
17.1
通讯作者：
Choo, Jaebum
中科院分区：
文献类型：
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作者：
Cheng, Ziyi;Choi, Namhyun;Choo, Jaebum
准确分析临床血清中的特异性生物标志物对于癌症的早期诊断和治疗至关重要。在这里，开发了一种基于表面增强拉曼散射（Sers）的免疫测定，使用磁珠和Sers纳米标签，用于测定游离与总（f/t）前列腺特异性抗原（PSA）的比率，以提高前列腺癌的诊断性能。为了评估所提出的方法的临床适用性，对4.0和10.0 ng/mL之间的灰色区域中的临床样本进行了基于SERS的测定，用于同时检测双重PSA标志物，游离PSA（f-PSA）和复合PSA（c-PSA）。我们的测定结果的f/t PSA比值显示出良好的线性相关性与使用电化学发光（ECL）系统安装在大学医院的临床实验室测量。此外，在13份临床血清样本中，同时测定法比平行测定法提供了更好的精密度。因此，我们的SERS为基础的分析，同时检测临床流体中的双重PSA标志物在前列腺癌的准确诊断中具有很强的应用潜力。
Accurate analysis of specific biomarkers in clinical serum is essential for early diagnosis and treatment of cancer. Here, a surface-enhanced Raman scattering (SERS)-based immunoassay, using magnetic beads and SERS nano tags, was developed for the determination of free to total (f/t) prostate specific antigen (PSA) ratio to improve the diagnostic performance of prostate cancer. To assess the clinical applicability of the proposed method, SERS-based assays for the simultaneous detection of dual PSA markers, free PSA (f-PSA) and complexed PSA (c-PSA), were performed for clinical samples in the gray zone between 4.0 and 10.0 ng/mL. Our assay results for f/t PSA ratio showed a good linear correlation with those measured using the electrochemiluminescence (ECL) system installed in the clinical laboratory of the University Hospital. In addition, the simultaneous assay provided better precision than parallel assays for the detection of f-PSA and c-PSA in 13 clinical serum samples. Therefore, our SERS-based assay for simultaneous detection of dual PSA markers in clinical fluids has strong potential for application in the accurate diagnosis of prostate cancer.