Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum.
Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum.
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DOI：
10.2174/1874091x00802010129
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Birkeland, Nils-Kare
中科院分区：
文献类型：
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作者：
Haugland, Gyri Teien;Innselset, Marte;Madern, Dominique;Birkeland, Nils-Kare
细胞分裂周期蛋白6（Cdc 6）同源物被认为参与DNA复制的起始过程。在本研究中，两个Cdc 6蛋白的古菌嗜酸热浆菌的生化特性进行了。TaCdc 6 -1和TaCdc 6 -2在溶液中均表现为单体，并且在体内大量表达。此外，与TaCdc 6 -2相比，TaCdc 6 -1显示出较强的自磷酸化能力，并且自磷酸化活性不受DNA或MCM的影响。
Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity is not affected by DNA or MCM.