Decreased number of circulating CD34+KDR+ cells in asymptomatic subjects with preclinical atherosclerosis
Decreased number of circulating CD34+KDR+ cells in asymptomatic subjects with preclinical atherosclerosis
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DOI：
10.1016/j.atherosclerosis.2006.02.041
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发表时间：
2007-03-01
期刊：
影响因子：
5.3
通讯作者：
Simon, Alain
中科院分区：
文献类型：
--
作者：
Chironi, Gilles;Walch, Laurence;Simon, Alain
目的：为了评估循环内皮祖细胞（CEPCs）是否可以被认为是心血管事件发生前的危险标志物，这一点比临床上明显的atherosclerosis（AS）更不确定。方法：采用流式细胞术测定84例未经治疗的无心血管疾病受试者每ml血液中的CD34（+）KDR（+）细胞数。通过超声检测颈动脉、腹主动脉和股动脉部位的动脉粥样硬化斑块，并将这三个部位中受斑块影响的部位数计数为0、1、2或3。此外，通过计算机超声成像测量两个颈总动脉段的内膜中层厚度（IMT）。在颈动脉、主动脉或股动脉斑块存在时，CD34（+）KDR（+）细胞数量减少48%、29%或30（p <0.001，0.05，0.05，与无菌斑的部位相比，在存在3个菌斑的部位与0个菌斑的部位相比，（p <0.01），但不随IMT三分位数的增加而改变。校正脆性风险评分，这也与CD34（+）KDR（+）细胞数量减少有关（p <0.001），使得CD34（+）KDR（+）细胞数量与斑块无关，颈动脉部位除外（p <0.01）。CEPC数量的减少可能参与动脉粥样硬化的临床前阶段，并为传统风险因素提供关于全球风险评估(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Objectives: To assess whether circulating endothelial progenitor cells (CEPCs) can be considered as a cardiovascular risk marker before event has occurred, that is less firmly established than in clinically overt atherosclerosis.Methods: Number of CD34(+)KDR(+) cell number per ml blood was measured by flow cytometry in 84 untreated subjects without cardiovascular disease. Atherosclerotic plaque was detected by ultrasound in carotid, abdominal aortic and femoral sites and the number of sites affected by plaque among these three sites was counted as 0, 1, 2 or 3. Additionally, intima-media thickness (IMT) was measured by computerized ultrasound imaging of both common carotid segments.Results: CD34(+)KDR(+) cell number decreased by 48, 29 or 30% in the presence of carotid, aortic or femoral plaque (p < 0.001, 0.05, 0.05, respectively) as compared to the absence of plaque and by 70% in the presence of three sites affected with plaque as compared with 0 site with plaque (p < 0.01) but did not change with increasing IMT tertiles. Adjustment for Framingham risk score, that was also associated with decreased CD34(+)KDR(+) cell number (p < 0.001), made CD34(+)KDR(+) cell number associations with plaque insignificant, except at the carotid site (p < 0.01).Conclusions: Reduced CEPC number may participate to preclinical stage of atherosclerosis and provide additional information to traditional risk factors as regards global risk assessment. (c) 2006 Elsevier Ireland Ltd. All rights reserved.