cDNA cloning and induction of the alcohol dehydrogenase gene (Adh1) of maize.
cDNA cloning and induction of the alcohol dehydrogenase gene (Adh1) of maize.
复制标题
玉米乙醇脱氢酶基因（Adh1）的 cDNA 克隆和诱导。
DOI：
10.1073/pnas.79.9.2981
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发表时间：
1982
影响因子：
11.1
通讯作者：
W. Peacock
中科院分区：
文献类型：
--
作者：
W. Gerlach;A. Pryor;E. Dennis;R. Ferl;M. Sachs;W. Peacock
Adh1是玉米基因组中编码乙醇脱氢酶(ADH；乙醇：NAD(+)氧化还原酶，EC 1.1.1.1)的两个基因之一。它们来自于从厌氧处理的玉米幼苗根中提取的mRNA。初步根据杂交-释放-翻译实验中获得的体外多肽的相对分子质量和电泳性进行鉴定。通过抗体沉淀和利用在Adh1基因座上具有不同遗传组成的玉米种质，证实了这种鉴定。测定了最长的cDNA片段的序列，约900个碱基对，编码168个COOH-末端氨基酸，3‘非翻译区为364个碱基对。反向Southern杂交表明，两个不同的Adh1-S群体产生1,650个核苷酸的基因，而三个Adh1-F群体产生另外一个1,750个核苷酸的基因。在厌氧过程中，Adh1的mRNA水平增加了50倍，在5小时达到最大值。返回到有氧条件下，厌氧诱导的Adh1 mRNA的半衰期超过18小时。
cDNA clones of Adh1, one of two genes encoding alcohol dehydrogenase (ADH; alcohol:NAD(+) oxidoreductase, EC 1.1.1.1) in the maize genome, have been isolated. They were derived from mRNA extracted from anaerobically treated roots of maize seedlings. Identification was initially made on the basis of molecular weight and electrophoretic properties of the in vitro polypeptide obtained in hybridization-release-translation experiments. The identification was confirmed by antibody precipitation and by the use of maize stocks having different genetic constitutions at the Adh1 locus. The sequence of the longest cDNA segment, approximately 900 base pairs, was determined and appears to code for 168 COOH-terminal amino acids and to have a 3' nontranslated region of 364 base pairs. Reverse Southern hybridizations established that two different Adh1-S stocks produce a mRNA of 1,650 nucleotides, whereas an additional mRNA of 1,750 nucleotides is produced in three Adh1-F stocks. A 50-fold increase in Adh1 mRNA level occurs during anaerobiosis, reaching a maximum at 5 hr. Return to aerobic conditions indicates a half-life of more than 18 hr for the anaerobically induced Adh1 mRNA.