Derepression of citrate synthase in Saccharomyces cerevisiae may occur at the level of transcription.
Derepression of citrate synthase in Saccharomyces cerevisiae may occur at the level of transcription.
复制标题
酿酒酵母中柠檬酸合酶的去抑制可能发生在转录水平。
DOI：
10.1128/mcb.4.2.247-253.1984
复制
发表时间：
1984
影响因子：
5.3
通讯作者：
Lewin,AS
中科院分区：
文献类型：
--
作者：
Hoosein,MA;Lewin,AS
通过全细胞脉冲追逐标记和无细胞蛋白质合成，证实了线粒体柠檬酸合成酶在酿酒酵母中是一个较大的前体。由于在体外可以用N-[35S]甲酰蛋氨酸标记，54,000个My前体形式似乎是主要的翻译产物。监测INS对柠檬酸合成酶的诱导作用。在可发酵(葡萄糖)和不可发酵(乙醇和甘油)碳源上生长的啤酒细胞。柠檬酸合成酶活性和免疫反应蛋白的数量增加了15倍以上。酿酒细胞在含葡萄糖的培养基上进入生长停滞期。这一增加与该酶的可翻译RNA的增加是平行的。当细胞生长在非发酵碳源上时，没有检测到柠檬酸合成酶及其mRNA的增加。这些结果表明，柠檬酸合成酶的释放可能发生在转录水平。
Pulse-chase labeling in whole cells and cell-free protein synthesis were used to establish that the mitochondrial enzyme citrate synthase is made as a larger precursor inSaccharomyces cerevisiae. A 54,000Mrprecursor form appeared to be a primary translation product since it could be labeled withN-[35S]formylmethionine in vitro. The induction of citrate synthase was monitored inS. cerevisiaecells grown on fermentable (glucose) and nonfermentable (ethanol and glycerol) carbon sources. The amount of citrate synthase activity and immune-reactive protein increased more than 15-fold asS. cerevisiaecells entered the stationary growth phase on glucose-containing medium. This increase was paralleled by an increase in translatable RNA for the enzyme. When cells were grown on a nonfermentable carbon source, no increase in either citrate synthase or its mRNA was detected. The results suggest that the release of citrate synthase from catabolite repression may occur at the level of transcription.