Carbohydrate binding properties of banana (Musa acuminata) lectin -: I.: Novel recognition of internal α1,3-linked glucosyl residues
Carbohydrate binding properties of banana (Musa acuminata) lectin -: I.: Novel recognition of internal α1,3-linked glucosyl residues
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DOI：
10.1046/j.1432-1327.2001.02148.x
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发表时间：
2001-05-01
期刊：
影响因子：
--
通讯作者：
Goldstein, IJ
中科院分区：
文献类型：
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作者：
Mo, HQ;Winter, HC;Goldstein, IJ
通过定量沉淀、沉淀的半抗原抑制和等温滴定量热法技术对香蕉（Musa acuminata）和密切相关的车前草（Musa spp.）的凝集素进行检查表明，它们是甘露糖/葡萄糖结合蛋白，优先选择这些糖的α-端基异构形式。两者都可以使用支链 α-甘露聚糖（酵母甘露聚糖）和 α-葡聚糖（糖原、葡聚糖和淀粉）生成沉淀素曲线，但不能使用仅包含 α1,4- 和 α1,6- 糖苷键（异地衣聚糖和支链淀粉）的线性 α-葡聚糖生成沉淀素曲线。新的观察发现，香蕉和车前草凝集素识别内部 α1,3 连接的葡萄糖残基，这些残基存在于线性多糖 elsinan 和 nigeran 中。来自豌豆和扁豆的伴刀豆球蛋白 A 和凝集素，以及甘露糖/葡萄糖结合凝集素，不会与任何这些线性 α-葡聚糖沉淀。作者认为，这是植物凝集素识别内部 α1,3-糖苷键的第一份报告。这些凝集素可能存在于各自水果的果肉中，并与淀粉复合。
Examination of lectins of banana (Musa acuminata) and the closely related plantain (Musa spp.) by the techniques of quantitative precipitation, hapten inhibition of precipitation, and isothermal titration calorimetry showed that they are mannose/glucose binding proteins with a preference for the alpha -anomeric form of these sugars. Both generate precipitin curves with branched chain alpha -mannans (yeast mannans) and alpha -glucans (glycogens, dextrans, and starches), but not with linear alpha -glucans containing only alpha1,4- and alpha1,6-glucosidic bonds (isolichenan and pullulan). The novel observation was made that banana and plantain lectins recognize internal alpha1,3-linked glucosyl residues, which occur in the linear polysaccharides elsinan and nigeran. Concanavalin A and lectins from pea and lentil, also mannose/glucose binding lectins, did not precipitate with any of these linear alpha -glucans. This is, the authors believe, the first report of the recogniton of internal alpha1,3-glucosidic bonds by a plant lectin. It is possible that these lectins are present in the pulp of their respective fruit, complexed with starch.